Comparison of sperm selection methods in intracytoplasmic injection.

A clinical trial to compare the effectiveness of routine method with hyaluronic acid and hypo-osmotic swelling test on sperm selection, fertilization evaluation, embryo development, embryo quality, and implantation rate in intracytoplasmic injection of oligoasthenozoospermic men.

Status

Recruiting

Phases

Phase 2

Study type

Interventional

Source

IRCT

Registry ID

IRCT2017090336039N1

Enrollment

Registered

2017-09-26

Start date

2017-10-07

Completion date

Unknown

Last updated

2018-02-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Oligoasthenospermia. Male infertility

Interventions

Intervention 1: Intervention group II: who is receiving hypo-osmotic swelling test. Intervention 2: Control group: who is receiving the routine sperm selection methods (i.e., sperm chromatin dispersio

Treatment - Other

Intervention group II: who is receiving hypo-osmotic swelling test.

Control group: who is receiving the routine sperm selection methods (i.e., sperm chromatin dispersion and microinjection).

Intervention group I: who is receiving hyaluronic acid (HA) binding.

Eligibility

Sex/Gender

All

Age

20 Years to 40 Years

Inclusion criteria

Inclusion criteria: Inclusion criteria: Males: age range of 22-40 years; infertility duration of 2-10 years; sperm count of 5×106-20×106 ml; and sperm motility rate of 25-40%. Females: aged range of 20-37 years; lack of infertility problem; and oocytes number of 6-7. Exclusion criteria: Males: no anomalies or spermatic leukocytes. Females: infliction with infertility problems (e.g., ovarian problems and endometriosis).

Exclusion criteria

Exclusion criteria:

Design outcomes

Primary

Measure	Time frame
Embryo development and quality. Timepoint: 3 days after intervention. Method of measurement: Grid system-Blastomere size and cytoplasm quality.;Evaluation of fertilization. Timepoint: 2 weeks after intervention. Method of measurement: Microscopic observation - The number of normally fertilized oocytes.;Implantation rate. Timepoint: 3 days after intervention. Method of measurement: Pregnancy pact observation by sonography and HCG test.	—

Measure

Time frame

Embryo development and quality. Timepoint: 3 days after intervention. Method of measurement: Grid system-Blastomere size and cytoplasm quality.;Evaluation of fertilization. Timepoint: 2 weeks after intervention. Method of measurement: Microscopic observation - The number of normally fertilized oocytes.;Implantation rate. Timepoint: 3 days after intervention. Method of measurement: Pregnancy pact observation by sonography and HCG test.

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Countries

Iran (Islamic Republic of)

Contacts

Public ContactMaryam Sefidgar Tehrani

Mashhad University of Medical Sciences

sefidgartm901@mums.ac.irEbrahimzadehBA@mums.ac.ir+98 51 3800 2486

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026