Effect of Vitamin D on Enzymes in type 2 Diabetes patients

Effect of Vitamin D on serum levels and gene Expression of Enzymes Allodose Reductase, Glucosamine Transferase and Glutamine Fructose Amidotransferase in Type 2 Diabetes Patients

Status

Active, not recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20170813035665N2

Enrollment

Registered

2019-06-12

Start date

2018-12-06

Completion date

Unknown

Last updated

2019-08-26

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Type 2 diabetes mellitus. Type 2 diabetes mellitus

Interventions

Intervention 1: Intervention group 1: receive 100 micro gram of vitamin D daily for 3 months. Intervention 2: Intervention group 2: receive 100 micro gram of placebo daily for 3 months.

Eligibility

Sex/Gender

All

Age

35 Years to 65 Years

Inclusion criteria

Inclusion criteria: Patients with type 2 diabetes who have35-65 years . patients take oral hypoglycemic drugs metformin or sulphonylurea. patients have been diagnosed with at least two years. Patients do not take supplemental drugs. patients with BMI less than 30 kg/m2

Exclusion criteria

Exclusion criteria: do not use vitamin D ,vitamin B6 supplements or diets at least 4-6 weeks before the intervention.

Design outcomes

Primary

Measure	Time frame
GFAT. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Aldose reductase. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Hexose amin transferase. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Glutamin Amido transferase gene expression. Timepoint: Before and three months after intervention. Method of measurement: Real time PCR.;Hexose amin transferase gene expression. Timepoint: Before and three months after intervention. Method of measurement: Real time PCR.;Aldose Reductase gene expression. Timepoint: Before and three months after intervention. Method of measurement: Real time PCR.	—

Measure

Time frame

GFAT. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Aldose reductase. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Hexose amin transferase. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Glutamin Amido transferase gene expression. Timepoint: Before and three months after intervention. Method of measurement: Real time PCR.;Hexose amin transferase gene expression. Timepoint: Before and three months after intervention. Method of measurement: Real time PCR.;Aldose Reductase gene expression. Timepoint: Before and three months after intervention. Method of measurement: Real time PCR.

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Secondary

Measure	Time frame
Weight. Timepoint: Before and three months after intervention. Method of measurement: seca scale in kilograms.;Waist. Timepoint: Before and three months after intervention. Method of measurement: Band meters in cm.;IL6. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Diastolic and systolic blood pressure. Timepoint: Before and three months after intervention. Method of measurement: Sphygmomanometer and Stethoscope will be used to measure the blood pressure.;BMI. Timepoint: Before and three months after intervention. Method of measurement: the formula for BMI is weight in kilograms divided by height in meters squared.;Insulin resistance. Timepoint: Before and three months after intervention. Method of measurement: HOMA-IR.;Vitamin D. Timepoint: Before and three months after intervention. Method of measurement: Electrochemiluminesence.;Calcium. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.;Insulin. Timepoint: Before and three months after intervention. Method of measurement: Electrochemiluminesence.;A1C. Timepoint: Before and three months after intervention. Method of measurement: Chromatoghraphy.;TNF. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Ages. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;PAI. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Ykl-40. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;TG. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.;Cholestrol. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.;HDL. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.;LDL. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.	—

Measure

Time frame

Weight. Timepoint: Before and three months after intervention. Method of measurement: seca scale in kilograms.;Waist. Timepoint: Before and three months after intervention. Method of measurement: Band meters in cm.;IL6. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Diastolic and systolic blood pressure. Timepoint: Before and three months after intervention. Method of measurement: Sphygmomanometer and Stethoscope will be used to measure the blood pressure.;BMI. Timepoint: Before and three months after intervention. Method of measurement: the formula for BMI is weight in kilograms divided by height in meters squared.;Insulin resistance. Timepoint: Before and three months after intervention. Method of measurement: HOMA-IR.;Vitamin D. Timepoint: Before and three months after intervention. Method of measurement: Electrochemiluminesence.;Calcium. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.;Insulin. Timepoint: Before and three months after intervention. Method of measurement: Electrochemiluminesence.;A1C. Timepoint: Before and three months after intervention. Method of measurement: Chromatoghraphy.;TNF. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Ages. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;PAI. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;Ykl-40. Timepoint: Before and three months after intervention. Method of measurement: Elisa.;TG. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.;Cholestrol. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.;HDL. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.;LDL. Timepoint: Before and three months after intervention. Method of measurement: Espectrophotometry.

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Countries

Iran (Islamic Republic of)

Contacts

Public ContactMahnaz zarei

Tehran University of Medical Sciences

mahnazzareei@yahoo.com+98 42933057

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026