Diabetes. Non-insulin-dependent diabetes mellitus
Conditions
Interventions
Intervention 1: Group (4) treatments with palmitate (500 µM) plus chicoric acid (50 µM), the cells of this group are treated with palmitate at a concentration of 500 µM for 24 hours. Then, they are tr
Treatment - Drugs
Group (4) treatments with palmitate (500 µM) plus chicoric acid (50 µM), the cells of this group are treated with palmitate at a concentration of 500 µM for 24 hours. Then, they are treated with chico
Separation of the cells is according to the ficole protocol. In the next step, cell pellets are suspended in the 2 cc culture medium and cell count evaluates using trypan blue. The cells are cultured
Group (3) treatment with chicoric acid at a concentration of 50 µM. The chicoric acid was dissolved in dimethyl sulfoxide and then reached the desired volume using the culture medium.
The cells of th
Group (2) treatment with palmitate with a concentration of 500 µM.
Sodium palmitate was dissolved in 50% ethanol at 55 °C. Then, using a culture medium containing bovine serum albumin 1% reached th
Sponsors
Hamadan University of Medical Sciences
Eligibility
Sex/Gender
All
Age
40 Years to 70 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria: FBS greater than 126 mg/dL; 2 hours blood glucose greater than 200 mg/dl; HbA1C greater than 6.5%; The control group should have a fasting blood glucose less than 100 mg/dl. Exclusion criteria: Use of medications such as antihypertensive drugs (such as beta-blockers), anti-diabetic drugs, estrogen supplements, thyroxine, diuretics and hypolipemic drugs; Other chronic and acute inflammatory diseases such as chronic kidney disease, cardiovascular disease, hypertension and ...; Infectious diseases, cancer, allergies; Other Endocrine Disorders (Except Type 2 Diabetes); Pregnancy; Smoking
Exclusion criteria
Exclusion criteria:
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| SIRT1. Timepoint: One day after the intervention, Two day after the intervention. Method of measurement: Gene expression of SIRT1 using Ct of gene.;IL-6. Timepoint: One day after the intervention, Two day after the intervention. Method of measurement: Gene expression of IL-6 using Ct of gene.;P-AMPK. Timepoint: One day after the intervention, Two day after the intervention. Method of measurement: concentration of p-AMPK using western blotting. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactNasrin Ziamajidi
Hamadan University of Medical Sciences
Outcome results
None listed