male infertility. Female infertility associated with male factors
Conditions
Interventions
Intervention 1: Preparation of semen sample using density gradient centrifugation by Hams F10+ HSA as washing medium. Intervention 2: Preparation of semen sample using density gradient centrifugation
Treatment - Other
Preparation of semen sample using density gradient centrifugation by Hams F10+ HSA as washing medium
Preparation of semen sample using density gradient centrifugation by Vita as washing medium
Preparation of semen sample using SPAS method
Semen sample without any action
Sponsors
Private
Eligibility
Sex/Gender
All
Age
20 Years to 40 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria: The number of sperms should be more than 15 million per mililiter; Sperm motility should be between 40 till 60 percent without a grade; Having at least one failed IUI. Exclusion criteria: Women with history of ovarian surgery; Endocrine disorders (polycystic ovarian syndrome, thyroid disorders, hyperprolactimenia, hypogonadotropic hypogonadism); Endometriosis; Age over 40 years.
Exclusion criteria
Exclusion criteria:
Design outcomes
Secondary
| Measure | Time frame |
|---|---|
| Occurrence of pregnancy. Timepoint: 2 weeks after intervention. Method of measurement: Mili IU per mililiter scale using beta hCG kit and Chemi Luminescence method. | — |
Primary
| Measure | Time frame |
|---|---|
| Sperm motility. Timepoint: 30 minutes after sample collection and immediately after intervention. Method of measurement: According to WHO criteria using the CASA software.;Sperm capacitation. Timepoint: 30 minutes after sample collection and immediately after intervention. Method of measurement: visual scale using chlortetracycline staining and fluorescence microscope.;Sperm DNA fragmentation. Timepoint: 30 minutes after sample collection and immediately after intervention. Method of measurement: visual scale using Halosprm kit and optical microscope.;Sperm viability. Timepoint: 30 minutes after sample collection and immediately after intervention. Method of measurement: visual scale using eosin nigrosin staining and optical microscope.;Sperm count. Timepoint: 30 minutes after sample collection and immediately after intervention. Method of measurement: According to WHO criteria using neubauer chamber and optical microscope. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactFaeze Davoodi
Iranian academic center for education, culture and research of Qom province
Outcome results
None listed