Overweight and obese. Overweight and obesity
Conditions
Interventions
Intervention 1: Intervention group: participants will observe Ramadan fasting for 4 weeks and, under the supervision of a dietitian, will receive a diet with a 20% caloric deficit consisting of 50% ca
each session will include 10 resistance exercises performed in a circuit format (2–3 sets of 10–12 repetitions per exercise), followed by 20–30 minutes of treadmill running at 60–65% of heart rate res
Sponsors
Shahrood University of Technology
Eligibility
Sex/Gender
Male
Age
18 Years to 55 Years
Inclusion criteria
Inclusion criteria: Age range 18 to 50 years Body mass index over 25 kg/m2 Inactive lifestyle
Exclusion criteria
Exclusion criteria: Having weight-loss diet Not permitted for exercise Use of antibiotic medications or probiotic supplements Presence of metabolic or gastrointestinal diseases including inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) Failure to adhere to the prescribed fasting protocol Failure to adhere to the exercise training protocol
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Gut firmicutes to bacteroidetes ratio. Timepoint: Measurement of stool firmicutes to bacteroidetes ratio before and after 4 weeks of intervention. Method of measurement: DNA extraction and analysis using 16S rRNA gene sequencing.;Short-chain fatty acids. Timepoint: Measurement of short-chain fatty acids before and after 4 weeks of intervention. Method of measurement: Chromatography.;Lipopolysaccharide-binding protein serum. Timepoint: Measurement of serum lipopolysaccharide-binding protein serum before and after 4 weeks of intervention. Method of measurement: Using a commercial kit through ELISA method.;Alpha diversity. Timepoint: Assessment of Alpha diversity of gut microbiota before and after 4 weeks of intervention. Method of measurement: Molecular sequencing and bioinformatics methods. | — |
Secondary
| Measure | Time frame |
|---|---|
| Weight. Timepoint: Measurement of weight before and after 4 weeks intervention. Method of measurement: Using digital scale.;Body fat percentage. Timepoint: Measurement of body fat percentage before and after 4 weeks intervention. Method of measurement: Bioempedance method by body composition analyzer InBody270.;High-density lipoprotein (HDL). Timepoint: Measurement of high-density lipoprotein before and after 4 weeks intervention. Method of measurement: Enzymatic colorimetric assay.;Fasting blood glucose. Timepoint: Measurement of fasting blood glucose before and after 4 weeks intervention. Method of measurement: Enzymatic colorimetric assay. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactFarhad Gholami
Shahrood University of Technology
Outcome results
None listed