obesity. Obesity due to excess calories
Conditions
Interventions
Intervention 1: Intervention groups: receive 50000 IU vit D as blues along with low calorie diet for 12 weeks. Intervention 2: Control group: receive placebo of vitamin D (edible paraffin) with low
Prevention
Intervention groups: receive 50000 IU vit D as blues along with low calorie diet for 12 weeks
Control group: receive placebo of vitamin D (edible paraffin) with low calorie diet for 12 weeks.
Sponsors
Nutrition Research Center, Tabriz University of Medical Scienses
Eligibility
Sex/Gender
All
Age
18 Years to 60 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria: age of 18-60; BMI= 30-40 Kg/m2; Serum Vitamin D < 50 nmol/L and interesting in participation in research. Exclusion criteria: Alcohol consumption; smoking; pregnant; lactating; menopausal women; very active subjectes or athletes; diet 3 mounts before research ; Glucose and lipid lowering Drugs ; antihypertensive medication; consumption vit D supplementation and multivitamin mineral during research; use of medication affecting Vitamin D metabolism; subjects suffering from metabolic disease ;cardiovascular diseases;renal diseases ; nephrolithiasis; liver diseases; thyroid disease; autoimmune disease; cancer.
Exclusion criteria
Exclusion criteria:
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Inflammatory factors (MCP-1, IL-1 beta). Timepoint: Beginning and end of the intervention. Method of measurement: ELISA Kit.;Serum Angiogenesis factors. Timepoint: Beginning and end of the intervention. Method of measurement: ELISA Kit.;Matrix metalloproteinases (MMP-2, MMP-9). Timepoint: Beginning and end of the intervention. Method of measurement: ELISA Kit.;Sirtuin-1 (Sirt-1). Timepoint: Beginning and end of the intervention. Method of measurement: ELISA Kit.;Toll like receptor-4 (TLR-4). Timepoint: Beginning and end of the intervention. Method of measurement: ELISA Kit. | — |
Secondary
| Measure | Time frame |
|---|---|
| Serum level of Vitamin D, calcium, Phosphorus and PTH hormon. Timepoint: Begining and end of intervention. Method of measurement: Vitamin D and PTH by using ELISA, calcium and Phosphorus by using spectrophotometry.;Fasting Blood Sugar (F.B.S.). Timepoint: Begining and end of intervention. Method of measurement: Enzymatic method.;Lipid profile (TC,LDL-c, HDL-c, TG). Timepoint: Begining and end of intervention. Method of measurement: Triglyceride, total cholesterol and HDL-cholesterol with enzymatic colorometric and LDL-cholesterol with Friedewald equation.;Serum insulin level and Insulin resistance. Timepoint: Begining and end of intervention. Method of measurement: insulin by using Elisa and Insulin resistance by Calculation of HOMA-IR.;Anthropometric measurements. Timepoint: Begining and end of intervention. Method of measurement: Weight and Height by using Seca scale and stadiometer respectively, waist to hip ratio will be calculated waist circumference divided to hip circumferenceas, Body mass index (BMI) will be calculated as weight in kilograms divided by the square of height in meters, fat mass and visceral fat mass by using body Analyser.;Energy , macro and micro nutrients intake. Timepoint: Baseline, 6 weeks and end of the intervention. Method of measurement: 3 day Dietary record questionnair.;Physical activity level. Timepoint: Baseline, 6 weeks and end of the intervention. Method of measurement: International physical activity questionnaire. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactSoodabeh Aliashrafi
Faculty of Nutrition and food science, Tabriz University of Medical Scienses
Outcome results
None listed