Effect of artificial oocyte activation on intra-Cytoplasmic sperm injection outcomes in specific infertile patients

Comparison of the effect of using or not using of artificial oocyte activation on intra-Cytoplasmic sperm injection outcomes in infertile patients with phospholipase C ? deficiency

Status

Active, not recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20160722029027N4

Enrollment

Registered

2018-01-21

Start date

2017-10-22

Completion date

Unknown

Last updated

2018-02-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Male infertility. Male infertility

Interventions

Intervention 1: Intervention= exposing oocytes in calcium ionophore A23187 following ICSI for 10 min and 10mM (sigma-aldrich). A23187 facilitate passing Ca iones from oocyte' s membrane. Intervention

Intervention= exposing oocytes in calcium ionophore A23187 following ICSI for 10 min and 10mM (sigma-aldrich). A23187 facilitate passing Ca iones from oocyte' s membrane.

Control= putting oocytes in culture medium following ICSI without calcium ionophore. The procedure is similar to conventional ICSI cycles.

Eligibility

Sex/Gender

All

Age

20 Years to 40 Years

Inclusion criteria

Inclusion criteria: couples subjected to ICSI cycles male factor infertility

Exclusion criteria

Exclusion criteria: any history of trauma and surgery in the testis age >40 years in men any female factor infertility such as endometriosis and poly cystic ovarian syndrome (PCOs) age >35 years in women

Design outcomes

Primary

Measure	Time frame
Fertilization rate. Timepoint: 3 days. Method of measurement: obsrevation with loop microscope.;Embryo cleavage. Timepoint: 3 days. Method of measurement: obsrevation with loop microscope.;Embryo quality. Timepoint: 3 days. Method of measurement: obsrevation with loop microscope.	—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactNahid Azad

Shahid Beheshti University of Medical Sciences

nazad1390@gmail.com+98 21 2387 2555

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026