The studied disease is repeated implantation failure(RIF).
Conditions
Interventions
Intervention group: People receiving peripheral blood mononuclear cells.
Sponsors
Tabriz University of Medical Sciences
Eligibility
Sex/Gender
Female
Age
30 Years to 40 Years
Inclusion criteria
Inclusion criteria: 1- All samples are from mothers aged 30-40 years. 2- All blood donors have no history of uterine problems (endometriosis, septate uterus), no ovarian problems (polycystic ovaries, weak ovarian reserve), no chromosomal abnormalities, no autoantibodies such as anti-TPO, anti-TG, ANA, APA, ACA, and anti-dsDNA. 3- BMI of people under 30 4- They are free of any uterine pathology. 5- The Siemens analysis and DNA fragmentation test of their spouses are normal.
Exclusion criteria
Exclusion criteria: 1- People aged less than 30 years or older than 40 years 2- Having ovarian problems (polycystic ovaries) 3- Presence of any uterine pathology (endometriosis, septate uterus) 4- Poor ovarian reserve (FSH>15 mIU/ml) 5- Having chromosomal abnormalities 6- Presence of autoantibodies such as anti-TPO, anti-TG, ANA, APA, ACA and anti-dsDNA. 7- The presence of mutations involving the coagulation system, such as factor XII, Pro C, Pro S8 deficiency - patients whose HIV, HCV or HBV test results are positive.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Counting of Th1 and Th2 in RIF patients - Counting of B cells in RIF patients - Counting of NK cells in RIF patients - Examination of autoantibodies panel Evaluation of pregnancy rate in patients with recurrent implantation failure Evaluation of live birth rate in RIF patient group Evaluation of abortion rate in women with RIF with low endometrial immune profile conditions. Timepoint: At the beginning of the study, counting of T and B lymphocytes as well as NK cells is done to identify people with low endometrial immunity, and in the next step, peripheral blood mononuclear cells are prescribed, and after 9 months, the rate of live births and abortions, as well as the pregnancy rate between the two intervention and control groups, are measured. Method of measurement: The method of counting lymphocytes and NK cells is with the help of flow cytometry, and the absence of autoantibodies is done with the help of ELISA. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactMehdi Yousefi
Tabriz University of Medical Sciences
Outcome results
None listed