Recurrent Pregnancy Loss(RPL).
Conditions
Interventions
Intervention group: The group of 200 patients with recurrent miscarriage was randomly divided into two groups of 100, one group receiving lymphocyte therapy and the other group not receiving treatment
Sponsors
Tabriz University of Medical Sciences
Eligibility
Sex/Gender
All
Age
18 Years to 40 Years
Inclusion criteria
Inclusion criteria: Having at least three consecutive abortions Having regular menstrual cycles BMI of people below 30 Have no uterine pathology Our patients will be selected only from Azerbaijan. Patients were selected whose semen analysis and DNA fragmentation test were normal.
Exclusion criteria
Exclusion criteria: Patients under the age of 18 and over 40 years Patients who themselves or their spouse have abnormal karyotypes or genetic or chromosomal abnormalities. Patients with chronic underlying disease who have to take certain medications. Patients with intrauterine anomalies. Having polycystic ovary syndrome Patients who test positive for HIV, HCV or HBV
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Frequency of Treg and Th17 cells. Timepoint: Before and after the intervention. Method of measurement: Flow cytometry.;Gene expression levels of Foxp3 and RORyt. Timepoint: Before and after the intervention. Method of measurement: Real-time PCR.;Protein expression levels of Foxp3 and RORyt. Timepoint: Before and after the intervention. Method of measurement: Western blot.;Gene expression levels of IL-10, IL-17, IL-21, IL-23, TGF-ß. Timepoint: Before and after the intervention. Method of measurement: Real-time PCR.;Secretion levels of IL-10, IL-17, IL-21, IL-23, TGF-ß. Timepoint: Before and after the intervention. Method of measurement: ELISA.;Gene expression levels of mir-146a ? mir-155 ? mir-326. Timepoint: Before and after the intervention. Method of measurement: real-time PCR. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactMehdi Yousefi
Tabriz University of Medical Sciences
Outcome results
None listed