?ffects of the relative expression of genes of sperm DNA methyltransferase on global methylation in patients with severe sperm abnormalities before and after folic acid uptake

Evaluating the relative expression and effects of methyltransferase on global genomic methylation rate in sperms of Oligoasthenoteratozoospermia patients with protamine deficiency before and after of folic acid uptake

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT201507285261N2

Enrollment

Registered

2016-03-27

Start date

2015-08-22

Completion date

Unknown

Last updated

2018-02-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Oligospermia. -

Interventions

Intervention 1: folic acid tablet in intervention group. Intervention 2: no intervention in control group.

Treatment - Drugs

folic acid tablet in intervention group

no intervention in control group

Eligibility

Sex/Gender

Male

Age

20 Years to 40 Years

Inclusion criteria

Inclusion criteria: Inclusion criteria includes severe sperm parameters and protamine disrupted, age less than 40, avoiding the use of drugs or alcohol, and varicocele Exclusion criteria: Unwillingness to continue during study,Diabetes,Blood Hypertension, obesity

Exclusion criteria

Exclusion criteria:

Design outcomes

Primary

Measure	Time frame
Sperm parameters including count, motility, morphology. Timepoint: Two months after the intervention. Method of measurement: Microscopic examination of sperm.;Relative gene expression of methyltransferase. Timepoint: Two months after the intervention. Method of measurement: PCR Real time.;Phosphate group condensation. Timepoint: Two months after the intervention. Method of measurement: Toloidine Blue.;Protamin deficiency. Timepoint: Two months after the intervention. Method of measurement: CMA3.	—

Measure

Time frame

Sperm parameters including count, motility, morphology. Timepoint: Two months after the intervention. Method of measurement: Microscopic examination of sperm.;Relative gene expression of methyltransferase. Timepoint: Two months after the intervention. Method of measurement: PCR Real time.;Phosphate group condensation. Timepoint: Two months after the intervention. Method of measurement: Toloidine Blue.;Protamin deficiency. Timepoint: Two months after the intervention. Method of measurement: CMA3.

—

Secondary

Measure	Time frame
Flatulence, insomnia. Timepoint: Two month after intervention. Method of measurement: Questionnaire.	—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactTahereh Rahiminia

Yazd Infertility Center

tahereh.rahiminia@gmail.com+98 35 3824 8343

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026