Comparison of clinical outcomes in tow sperm selection procedure

Comparison of clinical outcomes in two sperm selection procedures (density gradient centrifugation with surface markers- density gradient centrifugation) in infertile men intra-cytoplasmic sperm injection candidate

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT201504307223N3

Enrollment

Registered

2015-10-11

Start date

2014-01-28

Completion date

Unknown

Last updated

2018-02-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Male factor infertility. Male infertility

Interventions

Intervention 1: Intervention group: Half of oocytes were inseminated with sperm that selected with DGC following MACS processing (Separate intact spermatozoa from those that present apoptotic markers

Intervention group: Half of oocytes were inseminated with sperm that selected with DGC following MACS processing (Separate intact spermatozoa from those that present apoptotic markers by magnetic part

Internal control group: Remaining oocytes were inseminated with sperm that selected with DGC method (based on viability and morphology) (DGC group)

External control group: ?All the oocytes were inseminated by sperm that selected with DGC method

Eligibility

Sex/Gender

All

Age

20 Years to 45 Years

Inclusion criteria

Inclusion criteria: Inclusion criteria: Primary male factor infertility (at least, two of their semen parameters were considered as abnormal according to the WHO-2010) Exclusion criteria: Females factor infertility basely WHO criteria (2010) with higher than 35 years and less than 6 oocytes

Exclusion criteria

Exclusion criteria:

Design outcomes

Primary

Measure	Time frame
Sperm motility. Timepoint: In daye of ICSI procedure. Method of measurement: Using optical microscope, based WHO criteria.;Sperm concentration. Timepoint: In daye of ICSI procedure. Method of measurement: Using optical microscope, based WHO criteria.;Sperm morphology. Timepoint: In daye of ICSI procedure. Method of measurement: Using optical microscope, based WHO criteria.;Fertilization rate. Timepoint: 24 hours after ICSI procedure. Method of measurement: View of 2 pronucleuse with invert microscp.;Chemical pregnancy. Timepoint: 18 days after ICSI procedure. Method of measurement: Measurement of Beta HCG level in serum.;Quality of embryo. Timepoint: Three days post-oocyte retrieval. Method of measurement: Using a three-point scoring system including fragmentation rate, number of blastomere, shape and size.;Clinical l pregnancy. Timepoint: 12 weeks after ICSI procedure. Method of measurement: Fetal heart beat.	—

Measure

Time frame

Sperm motility. Timepoint: In daye of ICSI procedure. Method of measurement: Using optical microscope, based WHO criteria.;Sperm concentration. Timepoint: In daye of ICSI procedure. Method of measurement: Using optical microscope, based WHO criteria.;Sperm morphology. Timepoint: In daye of ICSI procedure. Method of measurement: Using optical microscope, based WHO criteria.;Fertilization rate. Timepoint: 24 hours after ICSI procedure. Method of measurement: View of 2 pronucleuse with invert microscp.;Chemical pregnancy. Timepoint: 18 days after ICSI procedure. Method of measurement: Measurement of Beta HCG level in serum.;Quality of embryo. Timepoint: Three days post-oocyte retrieval. Method of measurement: Using a three-point scoring system including fragmentation rate, number of blastomere, shape and size.;Clinical l pregnancy. Timepoint: 12 weeks after ICSI procedure. Method of measurement: Fetal heart beat.

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Countries

Iran (Islamic Republic of)

Contacts

Public ContactMohammad Hossein Nasr-Esfahani

Royan Institute (Royan Institute for Biotechnology)

mh_nasr@med.mui.ac.ir+98 31 9501 5688

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026