Recurrent spontaneous abortion. Spontaneous abortion
Conditions
Interventions
Intervention 1: Injection of husband or third donor lymphocytes to patients
Injection 80-85 × 106 husband peripheral blood mononuclear cells , dosage 0.5 ml, twice in a 3-week interval, subcutaneously
Treatment - Other
Injection of husband or third donor lymphocytes to patients
Injection 80-85 × 106 husband peripheral blood mononuclear cells , dosage 0.5 ml, twice in a 3-week interval, subcutaneously into forearms
Control group: No action
Sponsors
Sarem Cell Research Center, Tehran- Semnan University of Medical Sciences
Eligibility
Sex/Gender
Female
Age
20 Years to 39 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria:History of recurrent spontaneous abortion more than 3 times; Normal or negative to screening tests below Exclusion criteria:Anatomical abnormalities; Infectious disease such as TORCH; Genetic impairment; Abnormal karyotype of parents; Positive crossmatch test; Presence of autoimmune antibodies such as antiphospholipid antibodies, anti-nuclear antibodies and anti-thyroid antibodies; Thalassemia; Thyroid dysfunction
Exclusion criteria
Exclusion criteria:
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Alteration of cytokines secreted by Th17 cells. Timepoint: Beginning of study and day30. Method of measurement: ELISA method.;Alteration of cytokines secreted by Treg cells. Timepoint: Beginning of study and day30. Method of measurement: ELISA method. | — |
Secondary
| Measure | Time frame |
|---|---|
| Alteration of th17 cytokines in serum. Timepoint: Beginning of study and day 30. Method of measurement: ELISA method.;Alteration of regulatory Tcell secreted cytokines in serum. Timepoint: Beginning of study and day 30. Method of measurement: ELISA method. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactDr. Ahad Zare
Sarem cell research center, Sarem hospital
Outcome results
None listed