Infertility. Female infertility, unspecified
Conditions
Interventions
Intervention 1: Intervention group: Treatment group with calcium ionophore: Each oocyte is placed in a 30-ml droplet of ionophore calcium A23187 for 15 minutes, then washed in two drops of 30 µL of th
Sponsors
Shahid Beheshti University of Medical Sciences
Eligibility
Sex/Gender
All
Age
20 Years to 40 Years
Inclusion criteria
Inclusion criteria: Approval of the Ethics Committee of Shahid Beheshti University of Medical Sciences The age of a woman is between 20-40 years old Sperm has a normal parameter based on WHO criteria
Exclusion criteria
Exclusion criteria: Endometriosis Poly cystic ovary syndrome Genetic disorders
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Oocyte cytoplasmic maturation. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Cell Tracker Blue staining for oocyte cytoplasmic maturity.;Oocyte oxidative stress. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Evaluation of the free radical oxygen (ROS) in cytoplasmic by staining with H2DCFDA.;Meiotic spindle structure. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Evaluation of miieotic spindle structure by staining with anti-tubulin antibody.;Apoptosis rate. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Determination of apoptosis rate by evaluating expression bax, bcl2 and caspase-3 gene by Real Time-PCR.;Nucleation maturation. Timepoint: After 24-28 hours, the culture was incubated at 37 ° C and 5% CO2. Method of measurement: Determining the oocyte nucleus maturity by evaluating expression of MPF and MAPK by Real Time-PCR. | — |
Secondary
| Measure | Time frame |
|---|---|
| Fertilization rate. Timepoint: 16-18 hours after ICSI. Method of measurement: Divide the total number of oocytes fertilized to the total oocytes under ICSI.;Cleavage rate. Timepoint: The third day after ICSI. Method of measurement: Split the total number of cleavage embryos divided into fertilized embryos.;Embryo quility. Timepoint: The third day after ICSI. Method of measurement: Grading of embryo morphology based on the number and size of the blastomeres and the rate of fragmentation. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactElham Fazeli
Shahid Beheshti University of Medical Sciences
Outcome results
None listed