Adhesion of bacteria to fixed mandibular orthodontic retainer wires.
Conditions
Interventions
Intervention 1: Intervention group: dental bond from the lower right first premolar to the lower left first premolar with 0.175 inch retainer wires coated by titanium dioxide nanoparticles by vapor de
Sponsors
Hamedan University of Medical Sciences
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Patients who are in the debonding stage of orthodontics and only need a fixed retainer in the lower jaw Not using antibiotics and antibacterial mouthwashes during treatment No history of systemic diseases Not a smoker
Exclusion criteria
Exclusion criteria: The presence of missing teeth The presence of craniofacial syndromes and anomalies
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Adhesion of Streptococcus mutans and Lactobacillus bacteria to fixed mandibular retainer wires of orthodontic patients. Timepoint: 6 months after the intervention. Method of measurement: After the sixth month, the retainer wire between the canine teeth and the mandibular first premolar in both groups as control and control groups was taken out of the patient's mouth and transferred to the microbiology laboratory of the medical school to count the number of bacteria. Wires are stored in a sterile container containing phosphate buffer.Then, in order to separate the bacteria from the wires, 25% trypsin and ethylenediamine-theta-acetate solution are added to the samples and placed at the laboratory temperature for 45 minutes to separate the bacteria from the surface of the wire. In the next step, different dilutions are prepared from the resulting suspension and then inoculated in a plate containing MSA agar to detect and isolate Streptococcus mutans and incubated at 37 degrees Celsius for 24 hours.The samples are also inoculated on MRS culture medium (lactobacilli selective medium) and incubated at 37°C for 48 hours. Routine microbiological tests including gram staining, oxidase test, catalase activity, carbohydrate fermentation, and bile salt tolerance test will be used to determine Lactobacillus strains. Then the bacteria are counted and reported as CFU/mL. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactHossein sheikhi
Hamedan University of Medical Sciences
Outcome results
None listed