Type 2 diabetes mellitus. Non-insulin-dependent diabetes mellitus
Conditions
Interventions
Intervention 1: Intervention group: A 8-week low calorie diet ??)?based on subtracting 700 calories from standard formula to ?predict energy expenditure estimated at four levels of physical activity f
Treatment - Other
Intervention group: A 8-week low calorie diet ??)?based on subtracting 700 calories from standard formula to ?predict energy expenditure estimated at four levels of physical activity for obese women 1
Control group: without low calorie diet- Continuing with their common diet for 8-week
Sponsors
Tabriz University of Medical Sciences
Shahid Beheshti University of Medical Sciences
Eligibility
Sex/Gender
Female
Age
25 Years to 60 Years
Inclusion criteria
Inclusion criteria: The inclusion criteria: Women with type 2 diabetes diagnosed at least two years by specialist; The ?age range of 25 to 60; The degree of obesity in accordance with the definition of a BMI = 45 ?kg/m2 = 30; Willingness to participate in research.? The exclusion criteria: Patients who did not complete the questionnaire; Unwillingness to blood ?sampling; BMI over 45; Following a special diet like vegetarian diet, low calorie diet or weight-?loss medications for obesity last year; More than three kilograms of weight change over the past ?six months; Pregnancy, lactation, menstruation irregular; Chronic cigarette smokers (3 days more ?than 48 months) or alcohol; Vitamin and mineral supplements; protein and phytoestrogens period ?of 3 months prior to study entry; Taking hormonal contraceptives, anticonvulsants, ?glucocorticoids during the recent 3 months; Patients with type 1 diabetes mellitus; Kidney ?disease, liver disease, hyperthyroidism, a chronic inflammation (such as rheumatoid arthritis, ?multiple sclerosis, rash, etc.) and a variety of benign and malignant masses.?
Exclusion criteria
Exclusion criteria:
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Micro RNA-29a. Timepoint: before and 2 month after intervention. Method of measurement: qRT-PCR, copy nomber of micro RNAs per each micro litr of plasma and deffrent percent with interior standard.;Micro rna-375. Timepoint: before and 2 month after intervention. Method of measurement: qRT-PCR, copy nomber of micro RNAs per each micro litr of plasma and deffrent percent with interior standard.;Micro RNA-33b. Timepoint: before and 2 month after intervention. Method of measurement: qRT-PCR, copy nomber of micro RNAs per each micro litr of plasma and deffrent percent with interior standard.;Micro RNA-935. Timepoint: before and 2 month after intervention. Method of measurement: qRT-PCR, copy nomber of micro RNAs/ micro litr of plasma and deffrent percent with interior standard.;Fasting serum insulin concentration. Timepoint: before and 2 month after intervention. Method of measurement: ELISA, ?µU/mL.;Plasma insulin concentration 2 hours after meal. Timepoint: before and 2 month after intervention. Method of measurement: ELISA, ?µU/mL.;Serum adiponectin levels. Timepoint: before and 2 month after intervention. Method of measurement: ELISA, ng/dL.;Serum TNF-a levels. Timepoint: before and 2 month after intervention. Method of measurement: ELISA, ng/dL.;Fasting blood glucose. Timepoint: before and 2 month after intervention. Method of measurement: enzymatic colorimetric assay, mg/dL.;Blood glucose, blood glucose 2 hours after meal. Timepoint: before and 2 month after intervention. Method of measurement: enzymatic colorimetric assay, mg/dL.;Serum total cholestrol. Timepoint: before and 2 month after intervention. Method of measurement: enzymatic colorimetric assay, mg/dL.;Serum LDL-C. Timepoint: before and 2 month after intervention. Method of measurement: enzymatic colorimetric assay, mg/dL.;Serum HDL-C. Timepoint: before and 2 month after intervention. Method of measurement: enzymatic colorimetric assay, mg/dL.;Serum triglyceride level. Timepoint: before and 2 | — |
Secondary
| Measure | Time frame |
|---|---|
| Calorie intake. Timepoint: Before, 1 month and 2 month after intervention. Method of measurement: 3 day food recall and Food frequency questionnaire, Kcal/day.;Carbohdrate inake. Timepoint: Before, 1 month and 2 month after intervention. Method of measurement: 3 day food recall and Food frequency questionnaire, g/day.;Protein intake. Timepoint: Before, 1 month and 2 month after intervention. Method of measurement: 3 day food recall and Food frequency questionnaire, g/day.;Fat intake. Timepoint: Before, 1 month and 2 month after intervention. Method of measurement: 3 day food recall and Food frequency questionnaire,g/day. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactSomayeh Mohammadi
Tabriz University of Medical Sciences
Outcome results
None listed