Comparison of gene expression of immature oocytes before and after Ooplasmic transfer in Unexplained Infertile women in Mirza kochak Khan Hospital(2012-2013)

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT2013090512307N1

Enrollment

Registered

2013-09-05

Start date

2012-09-22

Completion date

Unknown

Last updated

2018-02-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

IVF, ART.

Interventions

Intervention 1: human Ooplasm Transfer 10-15% to immature oocytes and then oocytes culture for 24 hours in 5% co2 incubator. Intervention 2: control group 1,not intervention. Intervention 3: control g

human Ooplasm Transfer 10-15% to immature oocytes and then oocytes culture for 24 hours in 5% co2 incubator.

control group 1,not intervention

control group 2,oocytes culture for 24 hours in 5% co2 incubator

Eligibility

Sex/Gender

Female

Age

18 Years to 40 Years

Inclusion criteria

Inclusion criteria: Inclusion criteria: age 18-40 yrs; normal level of serum FSH/ LH/ PRL/ TSH; persistent mature and immature oocytes at OPU; long agonist protocol ovarian stimulation; mild endometriosis-Male Infertility-Un Explained Infertility-Tubal Factor. Exclusion criteria: PCO; moderate/severe Endometriosis and Genetic diseases.

Exclusion criteria

Exclusion criteria:

Design outcomes

Primary

Measure	Time frame
The effect of ooplasm transfer into immature oocytes on the gene expression of TGF-B family. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of cycle cell. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of apoptosis. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of ATPase 6 in mitcondria. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of DNA repairing. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of mitotic spindle. Timepoint: end of sampling. Method of measurement: Real Time PCR.	—

Measure

Time frame

The effect of ooplasm transfer into immature oocytes on the gene expression of TGF-B family. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of cycle cell. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of apoptosis. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of ATPase 6 in mitcondria. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of DNA repairing. Timepoint: end of sampling. Method of measurement: Real Time PCR.;The effect of ooplasm transfer into immature oocytes on the gene expression of mitotic spindle. Timepoint: end of sampling. Method of measurement: Real Time PCR.

—

Secondary

Measure	Time frame
The effect of ooplasm transfer into immature oocytes on the morphology after24 hrs oocyte incubation. Timepoint: 24 hrs after oocytes culture. Method of measurement: Invert Microscope.	—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactDr. Seyed Mohammad Hossein Noori Mugahi

Tehran University of Medical Sciences

Noorimoo@sina.tums.ac.ir+98 21 8895 3008

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026