Effect of sperm selection based on surface charge on pregnancy outcome after ICSI

Comparison of efficiency of density gradient centrifuge (DGC) with combined DGC/Zeta method on ICSI outcome.

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

IRCT

Registry ID

IRCT201108047223N1

Enrollment

500

Registered

2011-12-27

Start date

2008-04-08

Completion date

Unknown

Last updated

2018-02-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Male factor infertility. Male infertility

Interventions

Intervention 1: sperm selection based on Zeta method for ICSI procedure. Intervention 2: routine sperm selection method(density gradient centrifugation) for ICSI procedure in control group.

Treatment - Other

sperm selection based on Zeta method for ICSI procedure

routine sperm selection method(density gradient centrifugation) for ICSI procedure in control group

Eligibility

Sex/Gender

All

Age

20 Years to 45 Years

Inclusion criteria

Inclusion criteria: Inclusion criteria: infertility with male factor such as oligozoospermia, asthenozoospermia, tratozoospermia Exclusion criteria: infertility with female factor criteria, female's age more than 40 years

Exclusion criteria

Exclusion criteria:

Design outcomes

Primary

Measure	Time frame
Semen parameters. Timepoint: in day of ICSI procedure. Method of measurement: based on WHO 1999 criteria.;Level of sperm DNA fragmentation. Timepoint: in day of ICSI procedure. Method of measurement: using TUNEL test.;Fertilization rate. Timepoint: 24 hours After ICSI procedure. Method of measurement: view of 2 pronucleus with invert microscope.;Pregnancy. Timepoint: 20 day after ICSI procedure. Method of measurement: beta HCG level in serum.	—

Measure

Time frame

Semen parameters. Timepoint: in day of ICSI procedure. Method of measurement: based on WHO 1999 criteria.;Level of sperm DNA fragmentation. Timepoint: in day of ICSI procedure. Method of measurement: using TUNEL test.;Fertilization rate. Timepoint: 24 hours After ICSI procedure. Method of measurement: view of 2 pronucleus with invert microscope.;Pregnancy. Timepoint: 20 day after ICSI procedure. Method of measurement: beta HCG level in serum.

—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactMohammad Hossein Nasr Esfahani

Royan Institute

mh_nasr@med.mui.ac.ir+98 21 2356 2000

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Mar 1, 2026