Sperm selection procedure. Male infertility
Conditions
Interventions
Intervention 1: For each semen sample, two procedures will be carried out for sperm preparation. One of the procedures (Control) will be used for half of the sibling oocytes, and another procedure (st
Sweden).2. Reservoir chambers (2) – 4mls of G-Rinse (Vitrolife
Sweden) in each (8mls total). The selected sperm will then be collected from the harvest chamber based on the electric charge, and the sper
Sweden) will be transferred on 1ml of 80% PureSperm gradient (Vitrolife
Sweden) into a conical centrifuge tube. Then, 1ml of liquefied semen sample will be placed on the top of the upper layer, and the tube Will be centrifuged for 20 min at 1000rpm. The upper and lower la
Sponsors
Royan Institute
Eligibility
Sex/Gender
Female
Age
15 Years to 38 Years
Inclusion criteria
Inclusion criteria: Couples candidate for ICSI The number of follicles: at least 10 MII oocytes. Infertile couples with male factor infertility (sperm parameters lower than defined threshold by WHO 2021) Female age = 38 years Men with at least 2ml semen Embryo transfer only at blastocyst stage One blastocyst for transfer Normal female (female will be considered as normal if they have a regular cycle with patent tube and absence of endometriosis ) Men with a total sperm cell count higher than 5 million per ejaculate
Exclusion criteria
Exclusion criteria: Men with testicular sperm extraction or epididymal sperm aspiration Men with cryo-preserved semen samples
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Blastocysts rate and quality. Timepoint: Day 5 or 6 after ICSI. Method of measurement: Microscope. | — |
Secondary
| Measure | Time frame |
|---|---|
| Fertilization. Timepoint: 16-18 hours after ICSI. Method of measurement: The number of 2PNs divided by the number of MII oocytes injected by ICSI multiplied by 100.;Embryo quality. Timepoint: 2 or 3 day after ICSI. Method of measurement: According to DK Gardner criteria (2016).;Biochemical pregnancy. Timepoint: Post implantation. Method of measurement: Beta hCG level.;Clinical pregnancy. Timepoint: Fifth or sixth week of pregnancy. Method of measurement: The observation of a fetal sacs and also fetal heartbeat by sonography.;Implantation. Timepoint: One to two weeks after blastocyst transfer. Method of measurement: Sonography.;Livebirth. Timepoint: At the time of delivery. Method of measurement: By phone call. | — |
Countries
Iran (Islamic Republic of)
Contacts
Public ContactMohammad Hossein Nasr-Esfahani
Royan Institute
Outcome results
None listed