Evaluation of the effect of vitamin E on saliva of smokers

Evaluation and comparison of the effect of vitamin E on salivary total antioxidant capacity in smokers and non-smokers patients

Status

Active, not recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20110428006322N3

Enrollment

Registered

2021-03-05

Start date

2015-12-22

Completion date

Unknown

Last updated

2021-04-26

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

smoking , Total Antioxidant Capacity , Oral cancer prevention. Z58.7

Interventions

Intervention 1: Intervention group: Smokers between the ages of 30 and 70 years old who take a daily dose of 200 IU vitamin E product from Dana Drug Company orally for three weeks. Intervention 2: Con

Eligibility

Sex/Gender

All

Age

30 Years to 70 Years

Inclusion criteria

Inclusion criteria: Smokers with the history of 5 or more pack/year of smoking the smoker patients referred to Nader Kazemi Dental Clinic of Shiraz city

Exclusion criteria

Exclusion criteria: individuals with systemic diseases, dry mouth, history of oral ulcer or any inflammatory oral diseases, alcohol consumption, drug and medication use or supplemental vitamin consumption in the last three months patients with age over 65 years old

Design outcomes

Primary

Measure	Time frame
Total Antioxidant Capacity. Timepoint: Measurement of total salivary antioxidant capacity in the saliva sample of all participants was collected in the first stage before the intervention, 3 weeks after the intervention, then wash out and again 3 weeks later (equivalent to 6 weeks after the start of the intervention). And the measurement is done. Method of measurement: 20 ml of non-stimulated saliva in the sitting position during the period of 9 A.M to 12 A.M collected. The participants spitting in a 50ml. Falcon tube every 60 seconds. According to the status of the participants 5-15 minutes between each time spit break out. Sample stored at 0 to 5 ° C temperature. Samples were centrifuged (Sigma-Germany) at 4000 rpm. , 4 ° C for 10 minutes. Then centrifuged sample carried out by Pasteur Pipette (ISO-Lab, Germany) and placed in 2 ml tubes (ISAN Teb -Iran) in a freezer (Jal tajhiz-Iran) at -70 ° C. Fluorescence recovery after photobleaching (FRAP) technique used to measurement of total antioxidant capacity of samples. This method is based on the ability of saliva to recover ferric ions to the ferrite in the presence of a substance called TPTZ ( 2,4,6-Tri(2-pyridyl)-s-triazine), which is used as a reagent, resulting in a blue complex of TPTZ-Fe with a maximum absorption of 593 nm. The amount of salivary oxidation/reduction capacity measured by the concentration of the TPTZ-Fe blue complex by spectrophotometer at 593 nm.	—

Measure

Time frame

Total Antioxidant Capacity. Timepoint: Measurement of total salivary antioxidant capacity in the saliva sample of all participants was collected in the first stage before the intervention, 3 weeks after the intervention, then wash out and again 3 weeks later (equivalent to 6 weeks after the start of the intervention). And the measurement is done. Method of measurement: 20 ml of non-stimulated saliva in the sitting position during the period of 9 A.M to 12 A.M collected. The participants spitting in a 50ml. Falcon tube every 60 seconds. According to the status of the participants 5-15 minutes between each time spit break out. Sample stored at 0 to 5 ° C temperature. Samples were centrifuged (Sigma-Germany) at 4000 rpm. , 4 ° C for 10 minutes. Then centrifuged sample carried out by Pasteur Pipette (ISO-Lab, Germany) and placed in 2 ml tubes (ISAN Teb -Iran) in a freezer (Jal tajhiz-Iran) at -70 ° C. Fluorescence recovery after photobleaching (FRAP) technique used to measurement of total antioxidant capacity of samples. This method is based on the ability of saliva to recover ferric ions to the ferrite in the presence of a substance called TPTZ ( 2,4,6-Tri(2-pyridyl)-s-triazine), which is used as a reagent, resulting in a blue complex of TPTZ-Fe with a maximum absorption of 593 nm. The amount of salivary oxidation/reduction capacity measured by the concentration of the TPTZ-Fe blue complex by spectrophotometer at 593 nm.

—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactMostafa Rezaee

Shiraz University of Medical Sciences

rezaim@sums.ac.ir+98 71 3626 3192

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026