Study of the expression of genes involved in the process of death in oocytes in four groups of patients undergoing ovulation stimulation cycle with four therapies.

Study of the expression of genes involved in the process of apoptosis in cellulose cells in four groups of patients under the cycle of ovulation stimulation with antagonist or agonist GNRH with HP-FSH or FSH recombinant.

Status

Recruiting

Phases

Phase 3

Study type

Interventional

Source

IRCT

Registry ID

IRCT20101115005181N16

Enrollment

Registered

2020-06-12

Start date

2020-06-01

Completion date

Unknown

Last updated

2020-06-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Female Infertility. Female infertility associated with anovulation

Interventions

Intervention 1: The first intervention group: 20 infertile patients who were stimulated using the Gnrh agonist ampoule (Cinafact) of Cinagen Company, from the 21th day of previous cycle , 20 IU subcu

The eggs are aspirated from the follicular fluid and rinsed several times, then a drop of hyaluronidase is added to them and the cumulus is separated.Gene extract from Cumulus, At this stage, first R

Eligibility

Sex/Gender

Female

Age

20 Years to 30 Years

Inclusion criteria

Inclusion criteria: The age of 20- 30 years old. Normal response to induction of ovulation.

Exclusion criteria

Exclusion criteria: unexplained infertility Cause Azoospermia Ovarian hyper stimulation Syndrome

Design outcomes

Primary

Measure	Time frame
Maturation of Oocyte. Timepoint: 36 hours after the last administration of treatment. Method of measurement: Electronic Microscope.;Fertilization rate. Timepoint: 24 hours after intra cytoplasmic sperm injection. Method of measurement: Electronic Microscope.;Rate of Embryo development. Timepoint: The third day of intra cytoplasmic sperm injection. Method of measurement: Electronic Microscope.;The evaluation of Gene expression of HAS-2, BAX, BCL-2, VCAN, ALCAM, R PTGS-2 in cumulus of oocyte. Timepoint: The day of pick up ovum. Method of measurement: Real time PCR.	—

Measure

Time frame

Maturation of Oocyte. Timepoint: 36 hours after the last administration of treatment. Method of measurement: Electronic Microscope.;Fertilization rate. Timepoint: 24 hours after intra cytoplasmic sperm injection. Method of measurement: Electronic Microscope.;Rate of Embryo development. Timepoint: The third day of intra cytoplasmic sperm injection. Method of measurement: Electronic Microscope.;The evaluation of Gene expression of HAS-2, BAX, BCL-2, VCAN, ALCAM, R PTGS-2 in cumulus of oocyte. Timepoint: The day of pick up ovum. Method of measurement: Real time PCR.

—

Countries

Iran (Islamic Republic of)

Contacts

Public ContactSaeed Azizollahi

Tehran University of Medical Sciences

saeedazizollahi@yahoo.com+98 21 6658 1616

Outcome results

None listed

Source: IRCT (via WHO ICTRP) · Data processed: Feb 4, 2026