Physiological activation of sterile inflammatory pathways and the NLRP3 inflammasome by acute resistance exercise and six-month resistance-training adaptation in healthy adults.
Conditions
Interventions
Group 1: Healthy adults with no more than three months of resistance-training experience who intend to perform resistance training during the following six months. No training programme is prescribed.
V2 takes place within a maximum of 14 days and includes a pre-exercise blood sample, a standardized resistance-exercise session (leg press, leg curl, seated row, abdominal press, back extension and ch
3 sets of 10 repetitions at 70–80% of 1-RM, close to but not reaching complete exhaustion), and a blood sample approximately 10 minutes after exercise
V3 includes a blood sample 24 hours after V2. Self-selected training is documented weekly in REDCap.
Group 2: Healthy adults with more than one year of resistance-training experience who intend to con
V3 includes a blood sample 24 hours after V2. Self-selected training is documented weekly in REDCap
Sponsors
Universitätsklinikum Leipzig AöR, Klinik und Poliklinik für Kardiologie
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: Written informed consent; assignment to exactly one of the following groups: (1) no more than three months of resistance-training experience and intention to perform resistance training during the following six months; (2) more than one year of resistance-training experience and intention to continue resistance training during the following six months; or (3) currently no resistance training and no planned initiation of resistance training during the following six months.
Exclusion criteria
Exclusion criteria: Contraindication to maximal physical exertion according to the recommendations of the German Cardiac Society; lack of informed consent; known pregnancy; use of anabolic agents for performance enhancement; use of steroids.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Change in the serum concentration of circulating ASC specks (ASC specks/µL) from immediately before to approximately 10 minutes after the standardized resistance-exercise session, assessed at T0 and T1; comparison of changes between the three groups. | — |
Secondary
| Measure | Time frame |
|---|---|
| ASC speck concentration 24 hours after exercise and baseline ASC speck concentration; serum concentrations of IL-1ß, IL-6 and IL-18, creatine kinase and, where applicable, additional markers of sterile inflammation; VO2peak from cardiopulmonary exercise testing; muscle mass, fat-free mass and other bioelectrical impedance parameters; 1-RM for the six resistance exercises and change in maximal strength; documented training volume, frequency, duration, number of sets and repetitions, and training loads. Assessments are performed at T0 and T1, with blood sampling pre-exercise, approximately 10 minutes post-exercise and 24 hours post-exercise. | — |
Countries
Germany
Contacts
Public ContactSven Fikenzer
Universitätsklinikum Leipzig AöR, Klinik und Poliklinik für Kardiologie
Outcome results
None listed