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Next-generation sequencing (NGS) diagnostics in pneumonia

Next-generation sequencing (NGS) diagnostics in pneumonia

Status
Recruiting
Phases
Unknown
Study type
Observational
Source
DRKS
Registry ID
DRKS00040491
Enrollment
150
Registered
2026-07-03
Start date
2022-02-10
Completion date
Unknown
Last updated
2026-08-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

J18.9

Interventions

Group 1: Bronchoalveolar lavage fluid (BALF) and blood samples are collected from 150 patients with suspected or confirmed pneumonia requiring mechanical ventilation. These samples are used for standa

Sponsors

Klinik für Anästhesiologie und operative Intensivmedizin, Uniklinikum Bonn
Lead Sponsor

Eligibility

Sex/Gender
All
Age
18 Years to No maximum

Inclusion criteria

Inclusion criteria: Patients requiring mechanical ventilation with suspected or proven pneumonia

Exclusion criteria

Exclusion criteria: 1. Pregnancy 2. Age < 18 years old 3. Missing signed consent from the subject/patient or their legal representative to participate in the study and/or to the privacy policy.

Design outcomes

Primary

MeasureTime frame
The aim of this single center clinical observative trial is to evaluate the diagnostic performance (sensitivity, specificity, positive predictive value, negative predictive value, interrater-reliability using kappa statistics) of NGS-based diagnostics in comparison to culture and molecular based pathogen detection evaluating patients with suspected or proven pneumonia requiring mechanical ventilation. In addition, the present study will serve to retrospectively assess the clinical value of NGS-based diagnostics. For this purpose, an independent panel of experts will answer the question in each individual patient to what extent additional knowledge of the NGS results would have led to a change in the anti-infective therapy strategy.

Secondary

MeasureTime frame
Furthermore, it should be determined in which compartment (blood or BALF) NGS-based diagnostics demonstrate higher sensitivity and specificity for pathogen detection

Countries

Germany

Contacts

Public ContactChristian Bode

Klinik für Anästhesiologie und operative IntensivmedizinUniklinikum Bonn

christian.bode@ukbonn.de+4922828714119

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Aug 10, 2026