Evaluation of Cryobiopsy in the Colon: Quality of Obtained Samples, Depth of Penetration, and Optimal Freezing Time in Surgical Resection Specimens

Status

Recruiting

Phases

Unknown

Study type

Interventional

Source

DRKS

Registry ID

DRKS00037585

Enrollment

Registered

2025-07-30

Start date

2025-07-31

Completion date

Unknown

Last updated

2026-03-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

C18

Interventions

Group 1: Five cryobiopsies from each colonic resection specimen: – Three samples from the macroscopically normal periphery (with freezing times of 2, 3, and 4 seconds) – Two intratumoral samples (with

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: Age = 18 years Elective colonic resection for colorectal carcinoma Informed consent via Broad Consent for the use of biological samples

Exclusion criteria

Exclusion criteria: Tumor size < 2 cm in the resection specimen Refusal of Broad Consent

Design outcomes

Primary

Measure	Time frame
Biopsy size per sampling technique and freezing time	—

Secondary

Measure	Time frame
- Technical feasibility of cryobiopsy: Determined by the number of attempts required to obtain a sample per activation time. Up to three attempts may be made to define a biopsy as successful for a given activation time. – Serosal perforation – Histological quality (tissue architecture, proportion of submucosa, fragmentation) – Depth of tissue penetration in mm (measured pathologically) – Crush artifacts – Degree of dysplasia – Number of crypts – Assessment of architectural preservation – Deepest tissue layer reached – Exploratory: Histopathological tumor staging	—

Measure

Time frame

- Technical feasibility of cryobiopsy: Determined by the number of attempts required to obtain a sample per activation time. Up to three attempts may be made to define a biopsy as successful for a given activation time. – Serosal perforation – Histological quality (tissue architecture, proportion of submucosa, fragmentation) – Depth of tissue penetration in mm (measured pathologically) – Crush artifacts – Degree of dysplasia – Number of crypts – Assessment of architectural preservation – Deepest tissue layer reached – Exploratory: Histopathological tumor staging

—

Countries

Germany

Contacts

Public ContactAlexander Seeger

Universitätsmedizin, Medizinische Klinik 1, Schwerpunkt für Pneumologie und Allergologie

alexander.seeger@unimedizin-ffm.de069 6301 6336

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Mar 14, 2026