No health problem/disease - healthy adults are examined
Conditions
Interventions
Group 1: 48 hours of dry fasting
Group 2: 48 hours of water fasting
Sponsors
Charité - Universitätsmedizin Berlin, Charité-Hochschulambulanz für Naturheilkunde im Immanuel-Krankenhaus Berlin-Wannsee
Eligibility
Sex/Gender
All
Age
18 Years to 68 Years
Inclusion criteria
Inclusion criteria: - Body Mass Index (BMI) between 20 and 35 kg/m² - Ability to provide informed consent - Existing health insurance to allow for clarification and treatment of incidental findings - Willingness to complete both fasting interventions without interruption
Exclusion criteria
Exclusion criteria: - Eating disorders - Pregnancy or breastfeeding - Renal insufficiency - Pituitary insufficiency - Vaccination within 2 weeks prior to the start of the study intervention - Acute infection and/or antibiotic treatment within 2 weeks prior to the start of the study intervention - Symptoms or diagnosis of systemic inflammatory diseases or autoimmune diseases - Immunosuppression (including use of immunosuppressive medications) - Participation in ongoing or recently completed fasting interventions (e.g., alternate-day fasting, 5:2 diet, time-restricted eating) within 2 weeks prior to enrollment - Participation in other diet or weight loss programs (e.g., extensive sports training) - Night shift work or irregular working hours during the fasting intervention - Severe, manifest, and treatment-requiring diseases or functional disorders (internal, psychiatric, or neurological) that, in the opinion of the study physician, contraindicate participation - Ongoing use of prescription medications (except hormonal contraception) - Requirement for endocarditis prophylaxis - Postoperative recovery phases - Active smoking - Known medication, drug, or alcohol abuse - Legal incapacity or any other circumstances preventing understanding of the nature, significance, and scope of the study - Participation in another clinical study within the last two months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Homeostatic Model Assessment of Insulin Resistance HOMA-IR (Homeostatic Model Assessment of Insulin Resistance) comparison between water fasting and dry fasting to assess insulin sensitivity and glucose metabolism. At baseline and after 24 and 48 hours | — |
Secondary
| Measure | Time frame |
|---|---|
| Body composition (BIA): Measurement of body composition using bioelectrical impedance analysis (BIA) to assess fat mass, lean body mass, and changes before and after water and dry fasting. Time Frame: Baseline, and at Day 1 and Day 2 of fasting Anthropometric parameters: Measurement of Waist-to-Height Ratio and Waist-to-Hip Ratio to assess physical changes induced by water and dry fasting. Time Frame: Baseline, and at day 1 and day 2 of fasting Blood pressure: Monitoring of blood pressure to evaluate cardiovascular and respiratory effects of water and dry fasting. Time Frame: baseline and at day 1 and 2 of fasting Complete blood count: Analysis of differential blood count to assess hematological changes during fasting interventions. Time Frame: Baseline, Day 2 (after fasting) Blood lipids: Evaluation of blood lipids to investigate metabolic adaptations (measured in mmol/l) Time Frame: Baseline, Day 1 and Day 2 of fasting Inflammatory markers: Measurement of CRP, interleukins and cytokines to assess inflammation and immune response during fasting (measured in mmol/l) Time Frame: Baseline and Day 2 of fasting Coagulation parameter INR (International Normalized Ratio): Assessment of coagulation parameter INR Time Frame: Baseline, Day 2 of fasting Continuous glucose monitoring: Use of Freestyle Libre device to continuously monitor glucose levels before, during and after fasting interventions. (measured in mmol/l) Time Frame: Continuous during 14 days in a row including the 48-h fasting period Insuline-like growth factor 1 (IGF-1): Analysis of hormonal marker IGF-1 in serum to evaluate changes during fasting (measured in mmol/l). Time Frame: Baseline, and Day 1 and Day 2 of fasting Hypoxia-induced factors and antioxidant capacity: Measurement of hypoxia-induced factors (HIFs) and assessment of total antioxidant capacity (FRAP, DPPH assays) to analyze oxidative stress responses during fasting (measured in mmol/l) Time Frame: Baseline and Day 2 | — |
Countries
Germany
Contacts
Public ContactAndreas Michalsen
Charité - Universitätsmedizin Berlin,Charité-Hochschulambulanz für Naturheilkunde im Immanuel-Krankenhaus Berlin-Wannsee
Outcome results
None listed