C84.0 L41.8
Conditions
Interventions
Group 1: Parapsoriasis (PP)
The examinations in the study are carried out ex vivo using archived skin biopsies.
Single cell transcriptomics and single nuclear transcriptomics as well as additional im
Sponsors
Charité - Universitätsmedizin Berlin Klinik für Dermatologie, Venerologie und Allergologie
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: - Patients with a confirmed diagnosis of early-stage cutaneous T-cell lymphoma (mycosis fungoides type) - Patients with inflammatory skin diseases (eczema, psoriasis) and planned dermatosurgical intervention, where it is to be expected that non-lesional skin will also be excised - Patients with clinically typical small-plaque parapsoriasis (SPP) and histologically appropriate findings as well as a documented course of at least 6 months - Patients with clinically typical large-plaque parapsoriasis (LPP) and histologically appropriate findings and a documented course of at least 6 months
Exclusion criteria
Exclusion criteria: none
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| explorative study Aims are 1- the description and comparison of T cell populations in the skin and its microenvironment (TME) in parapsoriasis, early MF, patients with inflammatory dermatoses and healthy controls using single cell transcriptomics and spatial transcriptomics with subcellular resolution. Using Louvain clustering of gene expression data, we will identify similarities or differences in the expression of. >50,000 (single nucleus transcriptomics) genes per single nucleus. In a second step, it is possible to visualize cells with similar gene expression patterns directly on the histological section using spatial transcriptomics and to compare them with the results of single-nucleus transcriptomics as well as known histological criteria. 2- to investigate whether possible differences at the transcriptional level can also be confirmed at the protein level. For this purpose, immunohistochemical staining is performed on FFPE tissue. The investigations are carried out both on the tissue that has already been analyzed for single-cell transcriptomics and retrospectively on additional archived formalin-fixed tissue samples. | — |
Secondary
| Measure | Time frame |
|---|---|
| explorative study | — |
Countries
Germany
Contacts
Public ContactRose Moritz
Charité - Universitätsmedizin Berlin Klinik für Dermatologie, Venerologie und Allergologie
Outcome results
None listed