Isolation of peripheral blood mononuclear cells from patients with amnestic mild cognitive impairment (aMCI) for in vitro studies of nutritive agents.

Isolation of peripheral blood mononuclear cells from patients with amnestic mild cognitive impairment (aMCI) for in vitro studies of nutritive agents. - Peripheral energy metabolism in mild cognitive impairment / PEM-MCI

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

DRKS

Registry ID

DRKS00036017

Enrollment

Registered

2025-01-30

Start date

2022-03-09

Completion date

Unknown

Last updated

2025-04-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Mild cognitive impairment, dementia

Interventions

Group 1: People with amnestic mild cognitive impairment The patients were recruited from the memory clinic of the Department of Psychiatry, Psychosomatics, and Psychotherapy at the University Hospit

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: - Voluntary participation confirmed with signature (see patient or volunteer information and consent) - At least 18 years old - BMI over 18.5 kg/m2 - Memory performance at least 1.3 standard deviation below the norm (CERAD) - MMSE = 26

Exclusion criteria

Exclusion criteria: - Iron deficiency - Pregnancy/breastfeeding - Hemophilia - Taking anticoagulants - Blood donation in the last 8 weeks before the study day - Current presence of serious mental illness that could explain the memory deficit (major depressive episode, schizophrenia, severe substance abuse or dependence)

Design outcomes

Primary

Measure	Time frame
cognitive performance Various cognitive tests from the Consortium to Establish a Registry for Alzheimer's Disease (CERAD; German version) were used to assess cognitive performance. The CERAD test, which comprises a total of 11 subtests, includes five tests that are relevant for this study: The Animal Fluency Test (AFT) and the Semantic Fluency Test (SFT) measure executive functions and linguistic performance. They were combined into one “fluency” category. The Immediate Recall (IR) and the Delayed Recall (DR) both measure episodic memory over different time intervals. Finally, the Trail Making Test - Part B (TMT-B) was included to measure executive functions, in particular cognitive flexibility and attention.	—

Measure

Time frame

cognitive performance Various cognitive tests from the Consortium to Establish a Registry for Alzheimer's Disease (CERAD; German version) were used to assess cognitive performance. The CERAD test, which comprises a total of 11 subtests, includes five tests that are relevant for this study: The Animal Fluency Test (AFT) and the Semantic Fluency Test (SFT) measure executive functions and linguistic performance. They were combined into one “fluency” category. The Immediate Recall (IR) and the Delayed Recall (DR) both measure episodic memory over different time intervals. Finally, the Trail Making Test - Part B (TMT-B) was included to measure executive functions, in particular cognitive flexibility and attention.

—

Secondary

Measure	Time frame
mitochondrial function Mitochondrial function was assessed in peripheral blood mononuclear cells (PBMCs). The ATP content of the cells was determined using a luminescence kit (6016949, Perkin Elmer, Rodgau-Jügesheim, Germany). The activity of citrate synthase (CS) was measured by the conversion of DTNB to TNB in a self-developed assay. CS and ATP were measured using a ClarioStar Plate Reader (BMG Labtech, Ortenberg, Germany). To determine the complex activity of the respiratory chain, an Oxygraph-2k respirometer (Oroboros Instruments, Innsbruck, Austria) was used. All methods are also described in the following publication (doi:10.3791/65730).	—

Measure

Time frame

mitochondrial function Mitochondrial function was assessed in peripheral blood mononuclear cells (PBMCs). The ATP content of the cells was determined using a luminescence kit (6016949, Perkin Elmer, Rodgau-Jügesheim, Germany). The activity of citrate synthase (CS) was measured by the conversion of DTNB to TNB in a self-developed assay. CS and ATP were measured using a ClarioStar Plate Reader (BMG Labtech, Ortenberg, Germany). To determine the complex activity of the respiratory chain, an Oxygraph-2k respirometer (Oroboros Instruments, Innsbruck, Austria) was used. All methods are also described in the following publication (doi:10.3791/65730).

—

Countries

Germany

Contacts

Public ContactGunter Peter Eckert

Justus Liebiguniversität Gießen

eckert@uni-giessen.de06419939048

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 4, 2026