Neuropathological analysis of intraoperatively obtained, fragmented tumor tissue using aspirate histology

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

DRKS

Registry ID

DRKS00035570

Enrollment

250

Registered

2024-11-25

Start date

2024-11-28

Completion date

Unknown

Last updated

2025-04-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

C70.0 C71 C79 D37-D48 D10-D36 C81-C96

Interventions

Group 1: The aim of this project is to use the ultrasound aspirate for automated rapid histology - which in future should provide an alternative to the manual intraoperative frozen section diagnostics

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: Patients with CNS tumors who are to undergo brain tumor resection, for whom the UKSH has given broad consent or who have separately agreed to participate in the study, and in whom the ultrasonic aspirator is used.

Exclusion criteria

Exclusion criteria: none

Design outcomes

Primary

Measure	Time frame
Assessment of the feasibility and optimization of the procedure (dyeing time, type of dyes used, laser power, exposure time, scaling of the values in the subsequent image). Sensitivity/specificity of the procedure. During tumor resections on the CNS with the ultrasonic aspirator, resected tissue is produced as a waste product. This waste product consists mainly of tumor cells, blood, cerebrospinal fluid and irrigation fluid (Ringer's solution). This tumor aspirate is to be collected in the clinical study described here and examined for its suitability for neuropathological diagnosis using SLIDE microscopy. For this purpose, a tissue trap is placed between the suction tube of the ultrasonic aspirator and the collection container and part of the removed tissue (aspirate) is collected. This aspirate is then transferred to the SLIDE microscope to create a virtual staining and a virtual sectional image.	—

Measure

Time frame

Assessment of the feasibility and optimization of the procedure (dyeing time, type of dyes used, laser power, exposure time, scaling of the values in the subsequent image). Sensitivity/specificity of the procedure. During tumor resections on the CNS with the ultrasonic aspirator, resected tissue is produced as a waste product. This waste product consists mainly of tumor cells, blood, cerebrospinal fluid and irrigation fluid (Ringer's solution). This tumor aspirate is to be collected in the clinical study described here and examined for its suitability for neuropathological diagnosis using SLIDE microscopy. For this purpose, a tissue trap is placed between the suction tube of the ultrasonic aspirator and the collection container and part of the removed tissue (aspirate) is collected. This aspirate is then transferred to the SLIDE microscope to create a virtual staining and a virtual sectional image.

—

Secondary

Measure	Time frame
In addition to evaluating the measurement procedure (sensitivity, quality of image display), technical problems of SLIDE imaging in CNS tissue are to be investigated and, if possible, minimized by adapting the data acquisition with regard to image display and quality.	—

Countries

Germany

Contacts

Public ContactMatteo Mario Bonsanto

UKSH, Klinik für Neurochirurgie, Campus Lübeck

MATTEO.BONSANTO@UKSH.DE+4945150043211

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 4, 2026