Immunomodulation, monolayer repair and extracellular matrix remodelling of primary human AT-II cells during cyclic sinusoidal stretch

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

DRKS

Registry ID

DRKS00034350

Enrollment

Registered

2025-08-06

Start date

2026-06-01

Completion date

Unknown

Last updated

2026-03-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Tissue disruption/ regeneration lung

Interventions

Group 1: Extraction of cells from Lung tissue remains

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: - Consent to participate in the study - Planned (partial) lung resection due to central lung tumour or tumour in the upper lobe; transplantation or pneumothorax

Exclusion criteria

Exclusion criteria: - No consent to participate in the study or unable to give consent - Pneumonia

Design outcomes

Primary

Measure	Time frame
No autcomes for patients. Primary endpoints in vitro: - Release of IL-8 as indicator of the strength of the inflammatory response (target 1 and 3) - 2D migration speed; cell size; proliferation/apoptosis rate (target 2)	—

Secondary

Measure	Time frame
- Superoxide radicals, mitochondrial membrane potential, glutathione level, NF-kappaB synthesis, NF-kappaB translocation, NF-kappaB activity, expression of IL-1;IL-6 and IL-8, HMGB1, HSP60, HSP70, HSP96, NLRP3, collagen l; l; lll; lV; V and Vl, tropoelastin, fibrilin 1, fibulin 4 and 5 kinetics, integrin, MMP 2;8; 9; 12, hyaluronic acid, hyaluronidase activity, heparan sulafate proteoglycan; SP-A; SP-B; SP-C and SP-D - Migration distance, directedness, Rac1, cell size, aspect ratio, number of adherent and non-adherent cells, cytoskeleton, cell-cell and cell-matrix contacts, monolayer integrity, MTT test, KI-67, apoptosis/necrosis detection	—

Measure

Time frame

- Superoxide radicals, mitochondrial membrane potential, glutathione level, NF-kappaB synthesis, NF-kappaB translocation, NF-kappaB activity, expression of IL-1;IL-6 and IL-8, HMGB1, HSP60, HSP70, HSP96, NLRP3, collagen l; l; lll; lV; V and Vl, tropoelastin, fibrilin 1, fibulin 4 and 5 kinetics, integrin, MMP 2;8; 9; 12, hyaluronic acid, hyaluronidase activity, heparan sulafate proteoglycan; SP-A; SP-B; SP-C and SP-D - Migration distance, directedness, Rac1, cell size, aspect ratio, number of adherent and non-adherent cells, cytoskeleton, cell-cell and cell-matrix contacts, monolayer integrity, MTT test, KI-67, apoptosis/necrosis detection

—

Countries

Germany

Contacts

Public ContactSashko Spassov

Universitätsklinikum Freiburg

sashko.spassov@uniklinik-freiburg.de+4976127023080

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Mar 14, 2026