Healthy participants
Conditions
Interventions
Group 1: Food with 12 g Chlorella sorokiniana, 1 bread roll/day (12 g Chlorella sorokiniana per day) p.o., 14 days
Group 2: Food with 6 g Chlorella sorokiniana, 1 bread roll/day (6 g Chlorella sorokin
Sponsors
Technische Universität München, ZIEL - Institute for Food and Health, Lehrstuhl für Ernährung und Immunologie
Eligibility
Sex/Gender
All
Age
20 Years to 40 Years
Inclusion criteria
Inclusion criteria: Written declaration of consent; omnivore, vegetarian, vegan diet
Exclusion criteria
Exclusion criteria: Missing informed consent; current participation in another intervention study; known allergies or intolerances to Chlorella sorokiniana, the tested foods, mold, brillant blue FCF (E 133) or riboflavin (E 101); pregnancy or breastfeeding; body mass index below 18.5 or above 30 kg/m^2; special diets (low-carb, high-fat, formula, protein-supplemented, etc.); antibiotic treatment in the 6 months prior to the start of the study; diverticulitis; inflammatory bowel disease (ulcerative colitis, Crohn's disease); use of anticoagulants; pacemaker patients
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The changes in the composition of the microbiota of the participants after consumption of foods containing Chlorella sorokiniana (6 g or 12 g) are the primary endpoint of the study. To investigate this, stool samples are collected from the subjects throughout the study. This is done during the one-week wash-in phase, during the two-week intervention phase in which the bread rolls are consumed, and during the subsequent four-week follow-up phase. The stool samples are then examined using bacterial sequencing to assess any changes in the microbiota composition based on the sequencing results. The primary aim is to analyze the increase in bacterial diversity in the intestines of the participants after the consumption of foods enriched with Chlorella sorokiniana. | — |
Secondary
| Measure | Time frame |
|---|---|
| As a secondary endpoint of this study, the influence of the study foods on the metabolite profile in the intestines and blood of the participants will be examined to identify metabolic changes. For this purpose, the stool samples collected at the end of each phase (wash-in, intervention, follow-up) are analyzed for various metabolites (short-chain fatty acids, bile acids) using mass spectrometry. In addition, a blood sample is taken at the end of each phase. These blood samples are also analyzed for metabolites. | — |
Countries
Germany, Singapore
Contacts
Public ContactMadeleine Spohn
Technische Universität München, ZIEL - Institute for Food & Health, Lehrstuhl für Ernährung und Immunologie
Outcome results
None listed