AhR-dependent immune and inflammatory responses
Conditions
Interventions
Group 1: - plant-based diet according to standardized nutrition plan
- 3 male, 3 female
Group 2: - western diet according to standardized nutrition plan
- 3 male, 3 female
Sponsors
Charité - Universitätsmedizin Berlin
Eligibility
Sex/Gender
All
Age
18 Years to 50 Years
Inclusion criteria
Inclusion criteria: - Body mass index 18.5 - 29.9 kg/m2 - Stool frequency daily - existing health insurance - Taking L-thyroxine or iron supplements when TSH, Hb, MCV or MCH is allowed
Exclusion criteria
Exclusion criteria: - Autoimmune diseases - Chronic manifest diseases with or without permanent use of medication (e.g., diabetes, hypertension, hypercholesterolemia, psychiatric and neurological diseases, cardiovascular diseases with blood thinners, renal function impairment, chronic intestinal renal function, chronic intestinal diseases). - Postoperative phases - Acute infections - Malnutrition - Smokers (abstinence for at least 6 months) - Vegetarians - Vegan - Taking antibiotics within the last 3 months - Regular intake of food supplements (e.g. probiotics, creatine, protein powder, etc.) etc.) - Change in body weight of more than 2 kg in the previous month of the study - Pregnancy and lactation - Known medication, drug or alcohol abuse - Incapacity or other circumstances that do not allow the subjects to participate, fully understand the nature, meaning and implications of this study - claustrophobia - Illness, dysfunction, or other condition that, in the opinion of the investigator, precludes participation in the study. preclude participation in the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| - Measurement of in vitro activation of AhR in response to a Western vs. a plant-based diet over a 4-day period by incubation of AhR reporter cell lines with human serum. | — |
Secondary
| Measure | Time frame |
|---|---|
| - Change in gut microbiome composition measured in stool samples. - Change in gene expression of AhR-specific genes at the DNA, RNA and protein levels in various biological samples such as blood. | — |
Countries
Germany
Contacts
Public ContactJens Jordan
Deutsches Zentrum für Luft- und Raumfahrt (DLR), Institut für Luft- und Raumfahrtmedizin
Outcome results
None listed