Influence of fibers and polyphenols from sweet lupine and sunflower flour on the intestinal microbiome

Influence of fibers and polyphenols from sweet lupine and sunflower flour on the intestinal microbiome - BISS

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

DRKS

Registry ID

DRKS00028790

Enrollment

Registered

2023-02-22

Start date

2023-05-15

Completion date

Unknown

Last updated

2025-10-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy Participants

Interventions

Group 1: Food item with 15 g sunflower flour, 4 bars/day (60 g flour per day) p.o, 7 days Group 2: Food item with 15 g sweet lupine flour, 4 bars/day (60 g flour per day) p.o, 7 days Group 3: Food ite

Eligibility

Sex/Gender

All

Age

20 Years to 40 Years

Inclusion criteria

Inclusion criteria: Written informed consent; omnivorous diet

Exclusion criteria

Exclusion criteria: Lack of informed consent; current participation in other intervention studies; known allergies or intolerances towards ingredients of food items; pregnant or breastfeeding women; BMI 30 kg/m^2; vegetarian or vegan diet and other special diets (low-carb high-fat, formula, protein-enriched, etc.); treatment with antibiotics in the 6 months before the study; diverticulosis or diverticulitis

Design outcomes

Primary

Measure	Time frame
The primary outcome of this study is the altered composition of the participants’ microbiota upon consumption of food items containing sunflower and sweet lupine flour. Stool samples that were collected after consumption of one of these food items for one week will be analyzed via 16S rRNA gene analysis. Results will be compared to sequences obtained from stool samples collected after consumption of food with wheat flour for one week. Here, the primary goal is an increase of bacterial diversity (beta-diversity) in the participants’ colon after consumption of food items with either sunflower or sweet lupine flour.	—

Measure

Time frame

The primary outcome of this study is the altered composition of the participants’ microbiota upon consumption of food items containing sunflower and sweet lupine flour. Stool samples that were collected after consumption of one of these food items for one week will be analyzed via 16S rRNA gene analysis. Results will be compared to sequences obtained from stool samples collected after consumption of food with wheat flour for one week. Here, the primary goal is an increase of bacterial diversity (beta-diversity) in the participants’ colon after consumption of food items with either sunflower or sweet lupine flour.

—

Secondary

Measure	Time frame
The secondary outcome is the impact of food items containing sunflower or sweet lupine flour on the metabolite profile in the participants’ colon. Stool samples collected after consumption of these food items for one week will be analyzed via mass spectrometry to detect different metabolites (short chain fatty acids and secondary bile acids).	—

Countries

Germany

Contacts

Public ContactHelena Heimes

Technische Universität München, Lehrstuhl für Ernährung und Immunologie

helena.heimes@tum.de08161/712371

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 4, 2026