ketogenic diet, low-carb, immunonutrition, chronic inflammation, low-grade inflammation, longCOVID, autoimmunity
Conditions
Interventions
Group 1: Healthy volunteers with BMI 25 perform a three weeks/three month ad libitum ketogenic diet (KD).
Group 2: Healthy subjects after COVID-19 infection perform a three weeks/three month ad libitu
Sponsors
LMU Klinikum München. Klinik für Anästhesiologie
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: - written informed consent - age > 18 years Cohort 2 (additionally): - PCR-confirmed SARS-CoV-2 in the past (recovered) - Presence of one or more symptoms from the symptom complex of the Long-COVID syndrome Cohort 3 (additionally): - Presentation of one or more symptoms clinically considered to be associated with chronic inflammation and increased basal inflammatory T-cell function Cohort 4 according to Cohort 1
Exclusion criteria
Exclusion criteria: -Women who are pregnant or nursing -Application of glucocorticoids within the last 7 days -Serious metabolic or autoimmune disorders (such as diabetes) -Personal relationship with examiner. Cohort 3: -Women who are pregnant or nursing -Serious metabolic or autoimmune disorders (such as diabetes) -Personal relationship with examiner. Cohort 4: -Women who are pregnant or nursing -Application of glucocorticoids within the last 7 days -Personal relationship with examiner.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Changes of gene expression of pro-inflammatory cytokine IFNy in CD8+ T cells after three week KD. | — |
Secondary
| Measure | Time frame |
|---|---|
| Changes of additional pro and anti-inflammatory parameters: - mRNA expression profile in PBMC/whole blood: IL-2, IL-4, IL-6, IL-10, IL-17, IL-1beta, FOXP3, RORc, GATA3, T-bet, CTLA4 - protein secretion signature after TruCulture whole blood stimulation - Nextgene Sequencing for unknown pathways regulated by KD - flow cytometric analysis of T cell activation and composition subpopulations - analysis of mitochondrial respiration via flow cytometry (membrane potential, mitochondrial mass, ROS production), western-blot (protein levels of OXPHOS complexes, mTOR) - assessment of mitochondrial respiration and glycolysis as well as ATP production rate via Seahorse analysis - concentration of plasma ketone bodies via POCT - body weight and bioelectrical impedance analysis - comprehensive metabolomics, proteomics and transcriptomics using serum or immune cells - characterization of human microbiome (feces/urine) - analysis of amino acid pathways in urine samples (focus of tryptophan, phenylalanine) and bile metabolites in serum - assessment of health-related quality of life using WHOQOL-BREF/SF-36 questionnaire and Fatigue Assessment Scale - Analysis of the Disease Activity Score (DAS 28 CRP) - Analysis of the PASI score - Analysis of food-specific IgG/IgG4 Antibodies - Quantification of body height and weight and analysis of body composition using bioelectrical impedance analysis | — |
Countries
Germany
Contacts
Public ContactSimon Hirschberger
LMU Klinikum München, Klinik für Anaesthesiologie
Outcome results
None listed