U09.9
Conditions
Interventions
Group 1: post-covid syndrome group
To visualize and compare the lung microbiome with that of the control group, the DNA is first isolated from the sputum sample, prepared for sequencing, and then nex
Sponsors
MVZ Laborärzte Singen
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: Inclusion Criteria Post-Covid Syndrome Patients: - Age of majority - Diagnosed Post-Covid Syndrome (Which includes a previously contracted Covid-19 infection confirmed by PCR test). - a confirmed previously acquired Covid-19 infection (PCR test). - Furthermore, vaccinated and non-vaccinated patients will be included. Inclusion Criteria control group: - Age of majority - Vaccinated and non-vaccinated patients will be included.
Exclusion criteria
Exclusion criteria: Exclusion Criterion Post-Covid Syndrome Patients: - Positive PCR test at specimen collection (A PCR test will be performed from sputum at specimen collection to ensure that there is no active undetected infection. This is regulated by the informed consent). Exclusion Criteria Control Group: - Positive PCR test at specimen collection (A PCR test will be performed from the sputum at specimen collection to ensure that there is no active undetected infection. This is regulated by the consent form). - Previous knowingly confirmed passed Covid-19 infection (PCR test).
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Primary goal The lung microbiome of participants will be investigated using sputum samples. Accordingly, NO experiments will be performed on participants/patients (post-covid syndrome). Of interest is the change in the lung microbiome associated with post-covid syndrome compared to healthy participants (cohort analysis/prospective study). These changes will be shown by NGS. Primary outcome measure Difference of the microbiome of healthy subjects to the microbiome of patients suffering from post-covid syndrome, measured by alpha diversity. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary goal Furthermore, samples will be taken from post-covid syndrome patients multiple times (e.g. at the beginning of rehabilitation and at the end of rehabilitation) to show the recovery of the lung microbiome (longitudinal analysis/ prospective study). Secondary outcome measure To show is the normalization of the lung microbiome of post-covid syndrome patients over the period of rehabilitation in the direction of the control group, measured by alpha diversity. | — |
Countries
Germany
Contacts
Public ContactFolker Wenzel
Hochschule Furtwangen University, Fakultät Medical and Life Sciences
Outcome results
None listed