Perioperative characterization of the pulmonary microbiome in cardiovascular risk patients undergoing non-cardiac surgery - a monocentric cohort study.

Perioperative characterization of the pulmonary microbiome in cardiovascular risk patients undergoing non-cardiac surgery - a monocentric cohort study. - PERRIS

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

DRKS

Registry ID

DRKS00027141

Enrollment

120

Registered

2023-06-26

Start date

2024-01-22

Completion date

Unknown

Last updated

2025-11-24

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Cardiovascular diseases

Interventions

Group 1: Patients with an indication for surgery with a medium to high cardiovascular, procedure-specific risk and a Revised Cardiac Risk Index (RCRI) = 0 Group 2: Patients with an indication for surg

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: 1) Scheduled elective non-cardiac surgery with intermediate to high risk for cardiac complications 2) General anesthesia with endotracheal intubation 3) Signed informed consent of the participant

Exclusion criteria

Exclusion criteria: 1) Instable cardiac condition (Acute coronary syndrome, Acute heart failure, Tachyarrhythmia or Bradyarrhythmia, symptomatic Aortic stenosis) 2) Emergency surgery 3) Relative contraindication to perform a bronchoalveolar lavage (BAL) (Volume of air exhaled under forced conditions in the first second < 1000 mL, hypercapnia, hypoxemia, hemorrhagic diathesis) 4) Pregnancy 5) Breastfeeding

Design outcomes

Primary

Measure	Time frame
Concentration of bacterial DNA in copies/per mL of bronchoalveolar lavage measured by quantitative polymerase chain reaction after induction of general anesthesia.	—

Secondary

Measure	Time frame
• Number of unique bacterial species within a sample (alpha diversity, Shannon index). • Comparison of the composition of the microbiome between the different groups (beta diversity, Jaccard index). • Bacterial load, composition and diversity of the microbiome in the nasopharynx and comparison with the pulmonary microbiome. • ILC cell count and composition in the BAL and blood. • Inflammation markers (cellular and humoral) in the BAL and in the blood. • Cardiac markers (natriuretic peptides, cardiac troponin). • Frequency of perioperative complications based on the European Perioperative Clinical Outcome (EPCO) definition, including cardiovascular complications (MACE) (=major adverse cardiac and cerebrovascular event) • Severity of perioperative complications (classification based on the Clavien and Dindo classification). • Mortality (30, 60 and 180 days postoperative). • Need for and duration of treatment in an intensive care unit (days). • Duration of hospital treatment (days). • Necessity and duration of artificial respiration (hours). • Incidence of postoperative delirium. • Incidence of postoperative infections/sepsis (including urinary tract infection, pneumonia and acute respiratory distress syndrome). • Incidence of perioperative acute kidney injury. • Cardiac function using transthoracic echocardiography (TTE). • Cardiac function using magnetic cardiography (MCG). Change in health status (health-related quality of life) using patient-reported outcomes (PROs) • Survey using the standardized 5-dimensional-5-level EuroQoL (EQ-5D-L) questionnaire.	—

Measure

Time frame

• Number of unique bacterial species within a sample (alpha diversity, Shannon index). • Comparison of the composition of the microbiome between the different groups (beta diversity, Jaccard index). • Bacterial load, composition and diversity of the microbiome in the nasopharynx and comparison with the pulmonary microbiome. • ILC cell count and composition in the BAL and blood. • Inflammation markers (cellular and humoral) in the BAL and in the blood. • Cardiac markers (natriuretic peptides, cardiac troponin). • Frequency of perioperative complications based on the European Perioperative Clinical Outcome (EPCO) definition, including cardiovascular complications (MACE) (=major adverse cardiac and cerebrovascular event) • Severity of perioperative complications (classification based on the Clavien and Dindo classification). • Mortality (30, 60 and 180 days postoperative). • Need for and duration of treatment in an intensive care unit (days). • Duration of hospital treatment (days). • Necessity and duration of artificial respiration (hours). • Incidence of postoperative delirium. • Incidence of postoperative infections/sepsis (including urinary tract infection, pneumonia and acute respiratory distress syndrome). • Incidence of perioperative acute kidney injury. • Cardiac function using transthoracic echocardiography (TTE). • Cardiac function using magnetic cardiography (MCG). Change in health status (health-related quality of life) using patient-reported outcomes (PROs) • Survey using the standardized 5-dimensional-5-level EuroQoL (EQ-5D-L) questionnaire.

—

Countries

Germany

Contacts

Public ContactSascha Treskatsch

Klinik für Anästhesiologie mit Schwerpunkt operative Intensivmedizin | CBF, Charité - Universitätsmedizin Berlin

sascha.treskatsch@charite.de+49 30 450551522

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 4, 2026