The aim of our study is to investigate the effect of a short-term spermidine supplementation on inflammatory and immune system parameters as well as polyamine concentrations in blood of old and young adults.
Conditions
Interventions
Group 1: One group of "old adults" (n=10) aged 70 to 90 years and one group of "young adults" (n=10) aged 25 to 40 years take a spermidine supplement, as powder dissolved in water, three times per day
Sponsors
Deutsches Institut für Ernährungsforschung Potsdam-Rehbrücke (DIfE)
Eligibility
Sex/Gender
All
Age
25 Years to 90 Years
Inclusion criteria
Inclusion criteria: • Young adults: age: 25-40 years; BMI: 20-30 kg/m²; no chronic medication except for oral contraceptives; • Old adults: age: 70-90 years; BMI: 20-30 kg/m²; • No limitations in ‘’activities of daily living’’; • Subjects must be able to consent as defined by law.
Exclusion criteria
Exclusion criteria: • Pregnancy/breastfeeding; • Existing or past immune diseases such as leukemia, lymphoma, malignant neoplasms affecting bone marrow and lymphatic system, HIV or AIDS; • Self-reported severe or active medical conditions such as arteriosclerosis, advanced cardiac or respiratory disease, such as chronic obstructive pulmonary disease (COPD; GOLD-Stadium =3 (“Global Initiative for Chronic Obstructive Lung Disease”-classification)) or heart failure (NYHA =3 (“New York Heart Association“)); • Self-reported neurological or mental impairment; • Self-reported severe liver, kidney, or metabolic disease, such as liver cirrhosis (Child Pugh Score =2) or kidney disease (Stadium =3); • Drug/medication/alcohol dependence; • Existing polyamine supplementation; • Known intolerances or allergies to wheat germ, gluten, or histamine.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Investigate the effect of a spermidine intervention on inflammatory parameters by comparing concentrations of inflammatory parameters before and after an intervention with either a spermidine or a placebo supplement, as well as by comparisons between "young group" and "old group". Blood samplings occur before and after the 20-day intervention phase, and plasma, serum as well as blood cells will be extracted and stored at -80°C until laboratory analyses. Inflammatory parameters like C-reactive protein (CRP), tumor necrosis factor-a (TNF-a), interleukin 6 (IL-6) and parameters like Insulin-like growth factor 1 (IGF-1) in serum will be measured by commercial ELISA Kits. | — |
Secondary
| Measure | Time frame |
|---|---|
| Investigate the effect of a spermidine intervention on specific blood parameters and molecular processes by comparing concentrations before and after an intervention with either a spermidine or a placebo supplement, as well as by comparisons between "young group" and "old group". Blood samplings occur before and after the 20-day intervention phase, and plasma, serum as well as blood cells will be extracted and stored at -80°C until laboratory analyses. T-cell activity, autophagy and hypusine levels in PBMCs will be measured by molecular biological methods. Spermidine and polyamine concentrations in whole blood, plasma, serum and blood cells will be measured by LC-MS methods. Protein expression levels in whole blood will be measured by proteome analyses, so called proteomics. | — |
Countries
Germany
Contacts
Public ContactBastian Kochlik
Deutsches Institut für Ernährungsforschung Potsdam-Rehbrücke (DIfE)
Outcome results
None listed