Impact of a carbohydrate-reduced diet on the adaptive immunity state of healthy volunteers

Impact of a carbohydrate-reduced diet on the adaptive immunity state of healthy volunteers - Immuno keto nutrition

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

DRKS

Registry ID

DRKS00023373

Enrollment

Registered

2020-10-20

Start date

2019-09-16

Completion date

Unknown

Last updated

2025-04-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Ketogenic diet, low-carb nutrition, immunonutrition, chronic inflammation, low grade inflammation

Interventions

Group 1: Switch to a low carb diet over 3 weeks

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: Written informed consent Age = 18 years

Exclusion criteria

Exclusion criteria: Women during pregnancy and lactation Current intake of glucocorticoids Last intake of glucocorticoids > 7 days ago Severe metabolic disorder (e.g. diabetes mellitus) or Autoimmune diseases Participation in a clinical trial within the last 30 days Current participation in another research project or clinical trial Personal relationship with the examiner (relatives)

Design outcomes

Primary

Measure	Time frame
Alteration of mRNA expression of the proinflammatory cytokine IFNy in CD8+ T cells after a 3-week low-carb diet	—

Secondary

Measure	Time frame
Alteration of further inflammatory parameters (pro- and anti-inflammatory), mRNA expression profiles in PBMC and whole blood: IL-2, IL-4, IL-6, IL-10, IL-17, IL-1beta, FOXP3, RORc, GATA3, T-bet, CTLA4), Protein: TruCulture whole blood stimulation Change of inflammatory relevant mRNA and microRNA expression profiles by array analysis Flow cytometric analyses of T cell activation and subpopulations mitochondrial analyses: quantification, mitochondrial membrane potential, ROS production, protein quantification of respiratory chain complexes using Western blot, oxygen consumption and exratacellular acidification Ketone body concentrations in blood via POCT measurement Weight History Microbiological analysis from stool samples Analysis of the amino acid metabolic pathways (especially tryptophan, phenylalanine) in urine Analysis of bile acid metabolites in serum	—

Measure

Time frame

Alteration of further inflammatory parameters (pro- and anti-inflammatory), mRNA expression profiles in PBMC and whole blood: IL-2, IL-4, IL-6, IL-10, IL-17, IL-1beta, FOXP3, RORc, GATA3, T-bet, CTLA4), Protein: TruCulture whole blood stimulation Change of inflammatory relevant mRNA and microRNA expression profiles by array analysis Flow cytometric analyses of T cell activation and subpopulations mitochondrial analyses: quantification, mitochondrial membrane potential, ROS production, protein quantification of respiratory chain complexes using Western blot, oxygen consumption and exratacellular acidification Ketone body concentrations in blood via POCT measurement Weight History Microbiological analysis from stool samples Analysis of the amino acid metabolic pathways (especially tryptophan, phenylalanine) in urine Analysis of bile acid metabolites in serum

—

Countries

Germany

Contacts

Public ContactSimon Hirschberger

Klinikum der Universität München Klinik für Anästhesiologie und Intensivmedizin

simon.hirschberger@med.uni-muenchen.de089 44000

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 4, 2026