Detection of infections in ICU patients with digital holographic microscopy and magnetic flow cytometry in blood samples

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

DRKS

Registry ID

DRKS00023367

Enrollment

Registered

2020-11-23

Start date

2020-08-18

Completion date

Unknown

Last updated

2025-04-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Sepsis, SIRS, bacterial infection, viral infection, fungal infection non infectious diseases in ICU patients

Interventions

Group 1: ICU patients with infections: Analysis of heparinized blood samples with digital holographic microscopy and magnetic flow cytometry on up to four days with intervals of at least two days. In

current medication (antibiotics, antiplatelet drugs...)

SOFA Score and peak body temperature on the day of cytometry and the day after

microbiological findings regarding the day of cytometry (blood cultures, …) Group 2: ICU patients without infections Analysis of heparinized blood samples with digital holographic microscopy and magn

routine blood sample parameters (white blood cell count, procalcitonin/lactate/creatinine) on the day of cytometric measurement and the day after

microbiological findings regarding the day of cytometry (blood cultures, …)

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: Patients m/f = 18 years, assumed stay for = 48h in an ICU Patient having already a central venous line or an intraarterial catheter

Exclusion criteria

Exclusion criteria: pregnancy, refusal of consent of the patient or his/her person in charge lacking linguage skills in German or English of the Patient or his/her person in charge missing rountine blood test at the day of cytrometric analysis

Design outcomes

Primary

Measure	Time frame
Does the diagnosis of an infection correlate with changes in cell morphology in digital holographic microscopy and magnetic flow cytometry ? Possible morphological changes are compared with data from patients records. With these data we decide whether an infection was present on the day of analysis with holographic microscopy and magnetic flow cytometry. In case of an infection we document the underlying agent (bacteria, virus, mykosis)	—

Measure

Time frame

Does the diagnosis of an infection correlate with changes in cell morphology in digital holographic microscopy and magnetic flow cytometry ? Possible morphological changes are compared with data from patients records. With these data we decide whether an infection was present on the day of analysis with holographic microscopy and magnetic flow cytometry. In case of an infection we document the underlying agent (bacteria, virus, mykosis)

—

Secondary

Measure	Time frame
Is the used cytometry able to detect infections earlier than established blood sample parameters (procalcitonin/white blood cell count). Are the proven changes in cellmorpholgy more specific for infections than these. Is cytometry potentially a diagnostic tool to distinguish between the underlying agent (bacteria, virus, fungus)? Are possible changes in cell morphology only traceable in systemic bloodstream infections or also in localized ones.	—

Measure

Time frame

Is the used cytometry able to detect infections earlier than established blood sample parameters (procalcitonin/white blood cell count). Are the proven changes in cellmorpholgy more specific for infections than these. Is cytometry potentially a diagnostic tool to distinguish between the underlying agent (bacteria, virus, fungus)? Are possible changes in cell morphology only traceable in systemic bloodstream infections or also in localized ones.

—

Countries

Germany

Contacts

Public ContactHedwig Irl

Klinikum rechts der IsarKlinik für Anästhesie und Intensivmedizin

hedwig.irl@mri.tum.de089 4140 4291

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 4, 2026