The aim is to analyse circadian oscillations of the oral microbiome, including F. nucleatum, and the effects of oral hygiene measures on the oral microbiome composition over time.
Conditions
Interventions
Group 1: After an initial “wash-in” phase, biological material will be collected from the oral cavity during two separate time blocks. In the initial phase (day 0-4), subjects are asked to brush their
will be provided) and to avoid alcohol and tobacco products.
In Collection Block I (days 5-7), the volunteers are required to collect a saliva sample and to take a cheek swab every 4 hours for 2 conse
Sponsors
German Cancer Research Center (DKFZ)
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: Persons who have understood the informed consent and signed the written consent form.
Exclusion criteria
Exclusion criteria: - Rejection by the study participant - Pregnancy or nursing mothers - Lack of ability to consent - Persons who are currently taking or have taken antibiotics in the last 3 weeks - Infections of the oral mucosa, respiratory tract and GI tract in the last three weeks - Test persons with tumor diseases, or chronic inflammatory intestinal diseases - Subjects who are not able to fully consume diets orally - Underweight or obesity - Persons who received dentures or dental implants during the last 8 weeks - Dental treatments in the last 3 weeks
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Exploratory, longitudinal assessment of the diurnal variation of the relative abundance of Fusobacterium nucleatum as a member of the oral microbiota in a cohort of healthy volunteers with and without oral and dental hygiene. | — |
Secondary
| Measure | Time frame |
|---|---|
| - Characterization of oral microbiota using metagenome sequencing to determine the diurnal variation of species, microbial genes, and metabolic pathways. - Characterization of the stool microbiota at the end of each oral microbiota collection period. | — |
Countries
Germany
Contacts
Public ContactChristoph Stein-Thoeringer
German Cancer Research Center (DKFZ)
Outcome results
None listed