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Microbiological and pathological analysis of explanted Bone Flaps with different cryopreservation after decompressive hemicraniectomy

Microbiological and pathological analysis of explanted Bone Flaps with different cryopreservation after decompressive hemicraniectomy

Status
Active, not recruiting
Phases
Unknown
Study type
Observational
Source
DRKS
Registry ID
DRKS00023283
Enrollment
18
Registered
2020-10-26
Start date
2017-01-01
Completion date
Unknown
Last updated
2025-04-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

S06 I63

Interventions

Group 1: The study aims to examine and determine the microbiological condition, viability and the Infection potential of different cryopreserved Bone flaps (subgroup A: -20 Degree
Subgroup B: -80 degrees
Subgroup C: Computer-made Bone flaps, CAD).

Sponsors

Director, Department of Neurosurgery ,Katholisches Klinikum Lünen/Werne (KKLW)
Lead Sponsor

Eligibility

Sex/Gender
All
Age
18 Years to 100 Years

Inclusion criteria

Inclusion criteria: The patients, who have been in the Neurosurgical Clinic at the Catholic Klinikum Lünen / Werne Bone flaps explanted, which in the context of an Exitus letalis were not re-implanted.

Exclusion criteria

Exclusion criteria: none

Design outcomes

Primary

MeasureTime frame
The comparison of the microbiological status between explanted Bone flaps (subgroup A vs. subgroup B) using microbiological culture. The comparison of the bony microarchitecture of the explanted Bone flaps (subgroup A vs. subgroup B) using microscopy and the expression analysis of biomarkers of bone viability (parathyroid hormone type I receptor (PTH1R), osteoprotegerin (OPG), receptor of NFkB (RANKL), osteocalcin, C-terminal telopeptide) using immunohistochemistry (Subgroup A vs. subgroup B) in the explanted cranial bone (cortex and spongiosa)

Secondary

MeasureTime frame
The comparison of the infection potential after contamination of the explanted Bone flaps and the CAD with specific bacterial strains, i.e. whether pathogens grow at different speeds in the various subgroups. (Subgroup A vs. Subgroup B vs. Subgroup C)

Countries

Germany

Contacts

Public ContactRachit Agrawal

Neurosurgical Resident, Katholisches Klinikum Lünen/Werne ( KKLW)

agrawal.rachit@klinikum-luenen.de02306773151

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 18, 2026