E66.0 E66.9
Conditions
Interventions
Group 1: Patients with an independent of the OTB-study planned elective surgery in the clinic and polyclinic for oral and maxillofacial surgery or the clinic and polyclinic of otolaryngiology
Papillae biopsy is conducted during the planned surgery and under general anesthetic to minimize the patients effort
further planned tests do not differ between subjects from arm 1 and 2
Inclusion: subjects with obesity (BMI>=30) and normal weight (BMI=18.5-24.9)
no diagnosed impairement of gustatory and olfactory function
Group 2: Subjects without planned elective surgery who got interested due to public notices
Papillae biopsy is conducted under local anesthetic in the Polyclinic for Dental Prosthetics and Materials Science of the university clinic Leipzig
Inclusion: subjects with obesity (BMI>=30) or normal weight (BMI=18.5-24.9)
no impairement of gustatory and olfactory function
Sponsors
Medizinische Fakultät der Universität Leipzig
Eligibility
Sex/Gender
All
Age
18 Years to 69 Years
Inclusion criteria
Inclusion criteria: normal weight (BMI 18.5-24.9); obesity (BMI>=30); women and men; age: 18-69 years; signed consent
Exclusion criteria
Exclusion criteria: current pregnancy or currently breastfeeding, state after radiotherapy at head-neck area, state after or current chemotherapy, severe psychiatric-, or cardiact- or kidney-diseases, neurologic underlying diseases, use of antidepressants, anticonvulsants, steroids, immunosupressants, use of drugs, diagnosed T2D mellitus.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Transcriptom (RNA seq; single-cell seq) and epigenomanalyses (EPIC Array; ATAC seq) in isolated papillae fungiformes in obesity in contrast to normal weight for the identification of differential gene/protein-regulation; association analyses with factors of eating behavior (German version of the three factor eating questionnaire), with taste preferences (food preference questionnaire), with life style factors (diverse questionnaires), with data from taste screenings (taste strips) and with anthropometric and metabolic parameters from blood and saliva samples (f.e. cholestrol, blood sugar level, hormones/cytokines etc.) | — |
Secondary
| Measure | Time frame |
|---|---|
| functional studies using primary cell culture (isolated human cells from papillae fungiformes; analyses: f.e. Ca2+ imaging, in vitro knock down, stimulation of cell cultures with f.e. glucose etc.); Tongue- and saliva microbiome analyses; analysis of extracellular vesicles from saliva | — |
Countries
Germany
Contacts
Public ContactImke Schamarek
Klinik und Polyklinik für Endokrinologie, Nephrologie, Rheumatologie, Unversitätsklinikum Leipzig AöR
Outcome results
None listed