None listed
Conditions
Interventions
Group 1: The study contains two study arms with 8 subjects each. The participants of the first arm go through both study days in the pressure chamber with a pressure corresponding to a height of 8000
Sponsors
DLR- Deutsches Zentrum für Luft- und Raumfahrt - Institut für Luft- und Raumfahrtmedizin
Eligibility
Sex/Gender
All
Age
18 Years to 40 Years
Inclusion criteria
Inclusion criteria: - healthy - moderate physical activity / sports - Presence of written consent
Exclusion criteria
Exclusion criteria: - not the German language powerful - Rhinitis - chronic or acute lung diseases - Claustrophobia - BMI > or equal to 30 or 5 cups of coffee / black tea per day) - smoker - Pregnancy (early pregnancy test before each chamber exposure) - Menopause - no physical activity - intensive training within 1 week before the trial day - Disease with prolonged bed rest within 6 weeks before the trial day any other condition which, in the opinion of the performing physicians, makes the subject unsuitable (e.g., the ability to enter the pressure chamber independently) - no longer stay at altitudes over 1500 meters in the last 3 months - no flight longer than 1.5 hours in the last 6 weeks - no scuba diving within 3 months before the trial day - no regular singing exercises or playing wind instruments
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Determination of arterial blood oxygen saturation to verify that increased CO2 content of respiratory air under hypobaric conditions leads to an increase in blood oxygen saturation measured by: - Pulse oximetry - near-infrared spectroscopy (on head and thigh) - Capillary blood gas analysis (determination of partial pressures of oxygen (pO2) and carbon dioxide (pCO2), bicarbonate, base deficit and pH). During each test day, the test subjects are bled once before the test and then 6 times in each one-hour interval, from which all parameters. | — |
Secondary
| Measure | Time frame |
|---|---|
| - heart rate - spirometry (respiratory rate, breath / minute volume) - capnometry (etCO2) - Assessment of bone metabolism by means of: > Bone Formation Marker Procollagen Type 1 N-terminal Propeptide (P1NP)> Bone Resorption Marker Type 1 N-Telopeptide of Bone Collagen (NTx) > Biologically active calcium > Phosphate > Parathyroid hormone - Assessment of muscle metabolism by means of: > 3-methylhistidine in urine - Assessment of protein metabolism by: > Nitrogen balance in the urine - Assessment of the expression of the hypoxia-induced factor HIF1-alpha in leukocytes by means of: > Immunohistological stains > Enzyme-linked immunosorbent assay (ELISA) - Assessment of stress-related regulatory disorders using: > Cortisol - Measurement of activation of the transcription factor nuclear factor kappa light chain enhancer of activated B cells (NF-kB9) to draw conclusions about possible changes of the immune system - Assessment of the individual feeling (comfort) and harassment (shortness of breath, etc.) of the subjects: > Questionnaires following each test block - During each test day, the test subjects are bled once before the test and then 6 times in each one-hour interval, from which all parameters | — |
Countries
Germany
Contacts
Public ContactMartin Wittkowski
DLR - Institut für Luft- und Raumfahrtmedizin
Outcome results
None listed