healthy volunteers A41.9
Conditions
Interventions
Group 1: Full blood is collected from 20 healthy volunteers. Peripheral mononuclear blood cells (PMBCs) are examined for mitochondrial dysfunction after LPS stimulation during the time courses (after
Sponsors
Universitätsklinikum Knappschaftskrankenhaus Bochum
Eligibility
Sex/Gender
All
Age
18 Years to 65 Years
Inclusion criteria
Inclusion criteria: voluntary; healthy individuals age >18, <65, no risk for blood drawing
Exclusion criteria
Exclusion criteria: low body weight (65, anemia, anamnestic incompatibility of blood drawing
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Evaluation of the ratio of the mitochondrial and cytosolic concentration of TFAM beofre LPS stimulation and after LPS stimualtion during the time course (after 1/2, 4, 24, 48 hours) by western blot. | — |
Secondary
| Measure | Time frame |
|---|---|
| Evalutaion of the expression of mitochondrial mRNA, mRNA of TFAM and the regulation proteins (NFkB, PGC-1a, NRF1, NRF2) during the time course (after 1/2, 4, 24, 48 hours) by RT-PCR and Elisa. Furthermore the different TFAM Expression and distribution will be observerd in surivors compared to non-survivors after 30 days. | — |
Countries
Germany
Contacts
Public ContactTim Rahmel
Universitätsklinikum Knappschaftkrankenhaus Bochum GmbH Klinik fürAnästhesiologie, Intensivmedizin und Schmerztherapie
Outcome results
None listed