Experimentally induced contact eczema
Conditions
Interventions
Group 1: We will establish experimental contact eczema as chronic itch model in volunteers using diphencyprone. This model provides localized inflammatory ongoing itch that is ideally suited to study
Sponsors
"Medizinische Fakultät Mannheim der Universität HeidelbergExperimentelle Schmerzforschung"
Eligibility
Sex/Gender
All
Age
18 Years to 60 Years
Inclusion criteria
Inclusion criteria: Caucasian volunteers
Exclusion criteria
Exclusion criteria: Patients with chronic itch; Volunteers taking antipruritic drugs or having an infection at the potential biopsy area.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Morphological parameters: epidermal and dermal nerve fiber density, caliber deviations, branching, characterization of nerve fibers (myelin, neuropeptides, non-peptidergic markers, receptor expression). Functional parameters: pruritus intensity, spontaneous and evoked by electrical stimulation as well as diphencyprone application. Pruritus/pain intensity evoked by injection of lysophosphatidylic acid (LPA), BAM 8-22 (bovine adrenal medulla peptide 8-22), Beta-Alanin and Interleukin-31 after NGF sensitization. Immunostaining: expression of pruritic mediators. Gene expression: cutaneous global gene expression profiles, differential expression profiles. Microdialysis: lysophosphatidylic acid (LPA) analysis. | — |
Countries
Germany
Contacts
Public ContactRoman Rukwied
"Experimentelle Schmerzforschung, Medizinische Fakultät Mannheim der Universität Heidelberg"
Outcome results
None listed