CpG methylation is a relevant epigentic modification in sepsis

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

DRKS

Registry ID

DRKS00012965

Enrollment

Registered

2018-03-23

Start date

2017-12-01

Completion date

Unknown

Last updated

2025-04-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

healthy subjects

Interventions

Group 1: Full blood is collected from 20 healthy volunteers. Thrombocytes and peripheral mononuclear blood cells (PMBCs) are examined for epigenetic changes after LPS stimulation. An altered transloca

Eligibility

Sex/Gender

All

Age

18 Years to 65 Years

Inclusion criteria

Inclusion criteria: voluntary, healthy individuals age >18

Exclusion criteria

Exclusion criteria: low body weight, too youn age 65, anemia

Design outcomes

Primary

Measure	Time frame
Evaluation of a modified mitochondrial translation of the DNMT1 before LPS stimulation and after LPS stimulation during the time course (after ½, 2/4/8/24/48 hours) by Wester blot and immunofluorescence staining.	—

Secondary

Measure	Time frame
Evaluation of a modified methylation pattern on the cytosine of the mitochondrial DNA before LPS stimulation and after LPS stimulation during the time course (after ½, 2/4/8/24/48 hours) by immunofluorescence staining and genetic methylation analysis. In addition, a possible association between inflammation and anti-inflammation and epigenetic changes in the mtDNA is to be investigated.	—

Countries

Germany

Contacts

Public ContactTim Rahmel

Universitätsklinikum Knappschaftskrankenhaus Bochum

tim.rahmel@rub.de02342993001

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 4, 2026