Inflammatory parameters, redox status and microbiome. Healthy volunteers
Conditions
Interventions
Group 1: Vegan diet for 4 weeks:
The volunteers in this arm should emphasise a variety of plant foods and should not consume any animal products (e.g. dairy products, meat, fish, eggs or honey). Seve
Sponsors
Universitätsklinikum Freiburg, Institut für Umweltmedizin und Krankenhaushygiene, Uni-Zentrum Naturheilkunde
Eligibility
Sex/Gender
All
Age
18 Years to 60 Years
Inclusion criteria
Inclusion criteria: healthy, 18 - 60 years old, body mass index (BMI) between 21 kg/m^2 and 30 kg/m^2
Exclusion criteria
Exclusion criteria: pregnany and breastfeeding; regular intake of medication (except contraceptives or iodide); pronounced allergies (medication needed during allergy season); momentarily adhering to a vegan or vegetarian diet; aversion towards a meat-rich or vegan diet; eating disorder (anamnestically); drug, alcohol (>20g / day) or nicotine abuse (daily use), participation in another study or blood donation during the last four weeks.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| metabolic function of B-cells, measured by the sialylation of anti-bodies; count of regulatory T-cells, measured by the concentration of FoxP3; serum-concentration of Interleukin 10; redox status of immune cells, measured by the intracellular ROS-concentration In the beginning and in the end of the intervention we will take blood samples of all probands. Examination of the samples will be done in the end. | — |
Secondary
| Measure | Time frame |
|---|---|
| - CCR6, a chemokine-receptor on TH-17 cells - Neopterin, a marker of macrophages-activity - I?Ba-degradation - serum antibodies with galactosyl attachments - serum free light chains (? and ?) - serum IL-1, IL-6, IL-17, IL-20, IL-24, IL-27, TNF-a, TNF-ß, soluble IL-2 receptor - arachidonic acid and eicosanoids in urine - autophagy-markers (Atg8/LC3) - lymphocytes differentiation o activated T-cells: CD3+/HLA-DR+ o activated B-cells: CD19+/CD71+ (transferrin receptor) o natural regulatory T-cells: CD3+/CD4+/CD25+/CD127 low - differential blood count - Vitamin D (25(OH)D), Vitamin B12 (transcobalamin II) - Microbiological profile of gut flora - genome-wide methylation analysis - gene expression of cyclooxygenase in lymphocytes in peripheral blood In the beginning and in the end of the intervention we will collect samples of urine, stool and blood of all probands. Examination of the samples will be done in the end. | — |
Countries
Germany
Contacts
Public ContactManuel Hettich
Universitätsklinikum Freiburg, Institut für Umweltmedizin und Krankenhaushygiene, Uni-Zentrum Naturheilkunde
Outcome results
None listed