Patients suffering from chronic infections or passing trough server infections within the last 10 years
Conditions
Interventions
Group 1: blood withdrawal or apherese of patients who either have overcome severe infections or those natural immune system are able to control chronic infections stably
Group 2: blood withdrawal or a
Sponsors
Universitätsklinikum Köln
Eligibility
Sex/Gender
All
Age
18 Years to 65 Years
Inclusion criteria
Inclusion criteria: Patients suffering from chronic infections or passing trough severe infections within the last 10 years or healthy people as volunteers, and Karnofsky > 80
Exclusion criteria
Exclusion criteria: - Leukopenia 250 mL, a body weight of 50 kg is required) - 100 mL - Within 1 week after small surgery (biopsy, tooth extraction), or within 1 month after larger surgery (e.g., thoracotomoy, open abdominal surgery) or surgery with high risk of postoperative bleeding (e.g., tonsillectomy) - Acute or unknown health deterioration - Participation in another interventional study If a leukapheresis is planned, the following additional exclusion criteria apply: - History of significant complication from leukapheresis or citrate-based anticoagulation - INR > 1.5 or aPTT > 60 s - Premenopausal women that cannot rule out pregnancy at the time of apheresis
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Identification of neutralizing antibodies. Following methods are used: 1. isolation of B cells via flow cytometry ; 2. gene sequencing of antigen-coding central regions; 3. cloning and expression of identified genes; 4. functional analysis of rebuild antibodies using binding studies (ELISA, Biacore) and neutralization testings | — |
Countries
Germany
Contacts
Public ContactHenning Grüll
Institut für Virologie – Uniklinik Köln
Outcome results
None listed