Esophageal carcinoma C15
Conditions
Interventions
Group 1: Interventiongroup: The interventiongroup will have a single bout of aerobic exercise about 12 h befor tumorresection. The will performe a step-protocoll on a bycicle-ergometer. Load starts at
Sponsors
Deutsche Sporthochschule Köln, Institut für Kreislaufforschung und Sportmedizin, Abteilung für molekulare und zelluläre Sportmedizin
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: Esophageal carcinoma, befor Operation (tumoe removal), medical innocuousness for the sport intervention
Exclusion criteria
Exclusion criteria: Age > 18 years, therapy befor operatione (chemotherapy or radiation), medical contraindications for exercise
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Primary outcome is the nk-cell-cytotoxicity. Before, after and 12 h after the single bout of exercise blood will be drawn. From this blood samples nk-cells will be isolated and used in cytotoxicity assays with the cell line K562 as target cell. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary edpoints are the characterization of NK-cells and the tumorinfiltration by NK-cells. For the characterization blood will be drawn (befor, after and 12 h after exercise) and the NK-cells will be investigated for their subgroups (CD56bright and CD56dim) and for the degranulation marker CD107a by flow cytometry. The tumor tissue will be removed and analysed for NK-cell infiltration by imunohistochemistry. Therefore the NK-cells will be stained by CD56 and CD57. | — |
Countries
Germany
Contacts
Public ContactAlexander Schenk
Deutsche Sporthochschule Köln, Institut für Kreislaufforschung und Sportmedizin, Abteilung für molekulare und zelluläre Sprotmedizin
Outcome results
None listed