Genotyp-dependent metabolic changes provoked by nutritional challenges
Conditions
Interventions
Group 1: 1) Homozygous carriers of the minor allele of rs2014355 in the acetyl-CoA dehydrogenase, short chain (ACADS) locus are subjected to two metabolic challenge tests at two consecutive days. Afte
Sponsors
Else Kröner-Fresenius-Zentrum für Ernährungsmedizin
Lehrstuhl für Ernährungsmedizin
Technische Universität München
Eligibility
Sex/Gender
Male
Age
18 Years to 69 Years
Inclusion criteria
Inclusion criteria: 1. Subjects of the KORA-study tested for gene polymorphisms and with a normal OGTT result or disturbed glucose tolerance 2. Subjects between 18 and 69 years that are homozygous carriers of the minor alleles of rs2014355 and rs174547. 3. Subjects between 18 and 69 years that are homozgyous carriers of the major alleles of rs2014355 and rs174547. 3. Subjects with a BMI <35
Exclusion criteria
Exclusion criteria: 1. Known diabetes mellitus 2. Immunosuprressive therapy 3. Severe cardiovascular disease 4. Liver disease (GOT, GPT > 3-times of the norm) or kidney disease (cratinine > 1.2 mg/dl) 5. Psycic disease or inabillity to consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Identification of genotyp-specific differences in the metabolic changes provoked by different nutritional challenges to identify new biomarkers for an early and specific risc prediction. Therefore, clinical-chemical routine laboratory investigations in plasma samples, measurement of plasma metabolites (FIA-MS/MS, LC-MS/MS, GC-TOF-MS), measurement of eicosanoids in plasma (ELISA) and moleculargenetical studies in PBMCs for determination of proinflammatoric risc markers (PCR, western blots, EMSA) were applied. | — |
Secondary
| Measure | Time frame |
|---|---|
| Determination of metabolic changes provoked by nutritional challenges | — |
Countries
Germany
Contacts
Public ContactHans Hauner
Else Kröner-Fresenius-Zentrum für Ernährungsmedizin Lehrstuhl für Ernährungsmedizin Technische Universität München
Outcome results
None listed