Quantitative investigation of the activation of the cellular immune system by taste producing substances and 2-phenylethylamine from coffee, cocoa, diet lemonade, ginger tea, cabbage, and soy sauce.

Status

Recruiting

Phases

Unknown

Study type

Interventional

Source

DRKS

Registry ID

DRKS00005083

Enrollment

Registered

2019-09-11

Start date

2013-07-23

Completion date

Unknown

Last updated

2025-04-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

cellular immunsystem

Interventions

Group 1: This study consists of 6 parts according to the number of foodstuffs to be tested. After a wash-out-phase (5-7 days) the volunteers receive water as a control in the same amount as the foodst

Eligibility

Sex/Gender

All

Age

20 Years to 40 Years

Inclusion criteria

Inclusion criteria: healthy

Exclusion criteria

Exclusion criteria: taking medication, smoking, BMI >25, competetive sports, known metabolic disorders e.g. malabsorption syndrome, lipid dysfunctions, diabetes mellitus, thyroid dysfunction

Design outcomes

Primary

Measure	Time frame
activated leucocytes, genregulation of specific biomarkers (cytokines and their receptors) using ELISA, regulation of the expression of the particular chemosensory receptors (RT-qPCR).	—

Secondary

Measure	Time frame
Quantitative analytics of saccharin, caffeine, goitrin, 2-phenylethylamine, glutamate / piperonyl-n-heptylamine, and gingerol will be performed using stable-isotope-dilution analysis and LC-MS/MS. Plasma (100 µl) will be spiked with the respective isotope labelled internal standard. After equilibration samples will be diluted with acetonitrile, the precipitate will be centrifuged and the supernatant will be reduced in N2. After reconsitutation with H2O the solution will be analyzed by HPLC-MS/MS.	—

Measure

Time frame

Quantitative analytics of saccharin, caffeine, goitrin, 2-phenylethylamine, glutamate / piperonyl-n-heptylamine, and gingerol will be performed using stable-isotope-dilution analysis and LC-MS/MS. Plasma (100 µl) will be spiked with the respective isotope labelled internal standard. After equilibration samples will be diluted with acetonitrile, the precipitate will be centrifuged and the supernatant will be reduced in N2. After reconsitutation with H2O the solution will be analyzed by HPLC-MS/MS.

—

Countries

Germany

Contacts

Public ContactThomas Skurk

Else Kröner-Fresenius-Zentrum für Ernährungsmedizin an der Technischen Universität München Lehrstuhl für Ernährungsmedizin

skurk@tum.de08161-71 2007

Outcome results

None listed

Source: DRKS (via WHO ICTRP) · Data processed: Feb 4, 2026