A41.9
Conditions
Interventions
Group 1:
Patient:
1x 30 ml blood sample at 3 time points to analyse monocytes function, ChIP, Genexpression, cytokine,
in the course: messurement of HLA-DR
Group 2: Proband:
1x 30 ml blood sam
Sponsors
Universitätsklinikum Gießen und Marburg
Eligibility
Sex/Gender
All
Age
18 Years to 80 Years
Inclusion criteria
Inclusion criteria: patients: -age >= 18 years an = 18 years an <= 80 years
Exclusion criteria
Exclusion criteria: patients: -member of another study - dissonance with inclusion criteria - pregnancy - autoimmune disease - infectious viral disease - atherosclerosis with myocardial ischaemia in the past (e.g. infiltrate, anastomosis insufficiency) - renal failure - diabetes proband: -member of another study - dissonance with inclusion criteria - pregnancy - autoimmune disease - infectious viral or bacterial disease
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Genome-wide analysis of chromatin modifikation in isolated CD14+- monocytes of septic patients in early late and sustained sepsis. Chromatin Immunoprecipitation (ChIP) and Next Generation Sequencing (NGS) will be used. | — |
Secondary
| Measure | Time frame |
|---|---|
| -Genexpression CD 14+- monocytes (NGS) and cytockines (ELISA) in early, late and sustained sepsis -monocytic HLA-DR in the course of time (FACS) | — |
Countries
Germany
Contacts
Public ContactSebastian Weiterer
Klinik für Anaesthesiologie, Operative Intensivmedizin und Schmerztherapie Universitätsklinikum Gießen und Marburg, Standort Gießen
Outcome results
None listed